• Western blot analysis of extracts of various cell lines, using RhoGDI antibody (STJ115429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of extracts from normal (control) and RhoGDI knockout (KO) 293T cells, using RhoGDI antibody (STJ115429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ARHGDIA antibody (1-204) (STJ115429)

SKU:
STJ115429

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARHGDIA (1-204) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARHGDIA
Gene ID: 396
Uniprot ID: GDIR1_HUMAN
Immunogen Region: 1-204
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human RhoGDI (NP_001172006.1).
Immunogen Sequence: MAEQEPTAEQLAQIAAENEE DEHSVNYKPPAQKSIQEIQE LDKDDESLRKYKEALLGRVA VSADPNVPNVVVTGLTLVCS SAPGPLELDLTGDLESFKKQ SFVLKEGVEYRIKISFRVNR EIVSGMKYIQHTYRKGVKID KTDYMVGSYGPRAEEYEFLT PVEEAPKGMLARGSYSIKSR FTDDDKTDHLSWEWNLTIKK DWKD
Function Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.
Protein Name Rho Gdp-Dissociation Inhibitor 1
Rho Gdi 1
Rho-Gdi Alpha
Database Links Reactome: R-HSA-193634
Reactome: R-HSA-209563
Reactome: R-HSA-8980692
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013407
Reactome: R-HSA-9013408
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Rho Gdp-Dissociation Inhibitor 1 antibody
Anti-Rho Gdi 1 antibody
Anti-Rho-Gdi Alpha antibody
Anti-ARHGDIA antibody
Anti-GDIA1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance