Anti-ARHGAP44 antibody (666-772) (STJ29496)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ARHGAP44 (666-772) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ARHGAP44 |
| Gene ID | 9912 |
| Uniprot ID | RHG44_HUMAN |
| Immunogen Region | 666-772 |
| Immunogen Sequence | MADQSAGQPSPVSLSPTPPS TPSPYGLSYPQGYSLASGQL SPAAAPPLASPSVFTSTLSK SRPTPKPRQRPTLPPPQPPT VNLSASSPQSTEAPMLDGMS PGESMST |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 666-772 of human ARHGAP44 (NP_055674.4). |
Additional Info
| Tissue Specificity | Highly expressed in brain. Expressed at weak level in other tissues. |
| Function | GTPase-activating protein (GAP) that stimulates the GTPase activity of Rho-type GTPases. Thereby, controls Rho-type GTPases cycling between their active GTP-bound and inactive GDP-bound states. Acts as a GAP at least for CDC42 and RAC1. In neurons, is involved in dendritic spine formation and synaptic plasticity in a specific RAC1-GAP activity. Limits the initiation of exploratory dendritic filopodia. Recruited to actin-patches that seed filopodia, binds specifically to plasma membrane sections that are deformed inward by acto-myosin mediated contractile forces. Acts through GAP activity on RAC1 to reduce actin polymerization necessary for filopodia formation. In association with SHANK3, promotes GRIA1 exocytosis from recycling endosomes and spine morphological changes associated to long-term potentiation. |
| Protein Name | Rho Gtpase-Activating Protein 44Npc-A-10Rho-Type Gtpase-Activating Protein Rich2Rhogap Interacting With Cip4 Homologs Protein 2Rich-2 |
| Database Links | Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149 |
| Cellular Localisation | Cell ProjectionDendritic SpineRecycling EndosomePresynapseDendriteIn Ca1 Hippocampal SynapsesDetected At Both Presynaptic And Postsynaptic SitesLocated In Convoluted Dendritic Plasma Membrane Sections Enriched In Polymerized Actin And Myosin (Patches) Along Dendrites Where Often Emerge Filopodia |
| Alternative Antibody Names | Anti-Rho Gtpase-Activating Protein 44 antibodyAnti-Npc-A-10 antibodyAnti-Rho-Type Gtpase-Activating Protein Rich2 antibodyAnti-Rhogap Interacting With Cip4 Homologs Protein 2 antibodyAnti-Rich-2 antibodyAnti-ARHGAP44 antibodyAnti-KIAA0672 antibodyAnti-RICH2 antibody |
Information sourced from Uniprot.org