• Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g RhoGAP antibody (STJ11101814). Western blot was performed from the immunoprecipitate using RhoGAP antibody (STJ11101814) at a dilution of 1:500.
  • Western blot analysis of various lysates using RhoGAP Rabbit monoclonal antibody (STJ11101814) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ARHGAP5 antibody (1150-1300) [S4MR] (STJ11101814)

SKU:
STJ11101814

£212.50 - £501.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-RhoGAP (1150-1300) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARHGAP5
Gene ID: 394
Uniprot ID: RHG05_HUMAN
Immunogen Region: 1150-1300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1150-1300 of human RhoGAP (Q13017).
Immunogen Sequence: PSKYKYKSKTLFSKAKSYYR RTHSDASDDEAFTTSKTKRK GRHRGSEEDPLLSPVETWKG GIDNPAITSDQELDDKKMKK KTHKVKEDKKQKKKTKNFNP PTRRNWESNYFGMPLQDLVT AEKPIPLFVEKCVEFIEDTG LCTEGLYRVSG
Tissue Specificity Detected in skin fibroblasts (at protein level).
Function GTPase-activating protein for Rho family members.
Protein Name Rho Gtpase-Activating Protein 5
Rho-Type Gtpase-Activating Protein 5
P190-B
Database Links Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013405
Reactome: R-HSA-9013406
Reactome: R-HSA-9013408
Reactome: R-HSA-9013409
Reactome: R-HSA-9013423
Reactome: R-HSA-9035034
Reactome: R-HSA-9696264
Reactome: R-HSA-9696270
Reactome: R-HSA-9696273
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Also Membrane-Associated In A Fibrillar Pattern That Colocalizes With The Alpha5-Beta1 Integrin Receptor (Itga5/Itgb1) For Fibronectin
Alternative Antibody Names Anti-Rho Gtpase-Activating Protein 5 antibody
Anti-Rho-Type Gtpase-Activating Protein 5 antibody
Anti-P190-B antibody
Anti-ARHGAP5 antibody
Anti-RHOGAP5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance