Anti-ARHGAP44 antibody (430-510 aa) (STJ191325)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 430-510
STJ191325
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General Information

Short DescriptionRabbit polyclonal anti-Rho GTPase-activating protein 44 (430-510 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-2000
ELISA 1:5000-20000
FormulationLiquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolARHGAP44
Gene ID9912
Uniprot IDRHG44_HUMAN
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 430-510
Immunogen Region430-510 aa
SpecificityRHG44 Polyclonal Antibody detects endogenous levels of protein.

Additional Info

Function GTPase-activating protein (GAP) that stimulates the GTPase activity of Rho-type GTPases. Thereby, controls Rho-type GTPases cycling between their active GTP-bound and inactive GDP-bound states. Acts as a GAP at least for CDC42 and RAC1. In neurons, is involved in dendritic spine formation and synaptic plasticity in a specific RAC1-GAP activity. Limits the initiation of exploratory dendritic filopodia. Recruited to actin-patches that seed filopodia, binds specifically to plasma membrane sections that are deformed inward by acto-myosin mediated contractile forces. Acts through GAP activity on RAC1 to reduce actin polymerization necessary for filopodia formation. In association with SHANK3, promotes GRIA1 exocytosis from recycling endosomes and spine morphological changes associated to long-term potentiation.
Protein Name Rho Gtpase-Activating Protein 44
Npc-A-10
Rho-Type Gtpase-Activating Protein Rich2
Rhogap Interacting With Cip4 Homologs Protein 2
Rich-2
Database Links Reactome: R-HSA-8980692
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Cellular Localisation Cell Projection
Dendritic Spine
Recycling Endosome
Presynapse
Dendrite
In Ca1 Hippocampal Synapses
Detected At Both Presynaptic And Postsynaptic Sites
Located In Convoluted Dendritic Plasma Membrane Sections Enriched In Polymerized Actin And Myosin (Patches) Along Dendrites Where Often Emerge Filopodia
Alternative Antibody Names Anti-Rho Gtpase-Activating Protein 44 antibody
Anti-Npc-A-10 antibody
Anti-Rho-Type Gtpase-Activating Protein Rich2 antibody
Anti-Rhogap Interacting With Cip4 Homologs Protein 2 antibody
Anti-Rich-2 antibody
Anti-ARHGAP44 antibody
Anti-KIAA0672 antibody
Anti-RICH2 antibody

Information sourced from Uniprot.org

Citations

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