Anti-ARHGEF1 antibody (162-211 aa) (STJ95453)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human ARHGEF1 at the amino acid range 162-211
General Information
| Short Description | Rabbit polyclonal anti-Rho guanine nucleotide exchange factor 1 (162-211 aa) for use in WB, ELISA and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ARHGEF1 |
| Gene ID | 9138 |
| Uniprot ID | ARHG1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human ARHGEF1 at the amino acid range 162-211 |
| Immunogen Region | 162-211 aa |
| Specificity | RhoGEF p115 Polyclonal Antibody detects endogenous levels of RhoGEF p115 protein. |
Additional Info
| Post Translational Modifications | Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2. |
| Function | Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. In lymphoid follicles, may trigger activation of GNA13 as part of S1PR2-dependent signaling pathway that leads to inhibition of germinal center (GC) B cell growth and migration outside the GC niche. |
| Protein Name | Rho Guanine Nucleotide Exchange Factor 1115 Kda Guanine Nucleotide Exchange FactorP115-RhogefP115rhogefSub1.5 |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106 |
| Cellular Localisation | CytoplasmMembraneTranslocated To The Membrane By Activated Gna13 Or Lpa Stimulation |
| Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor 1 antibodyAnti-115 Kda Guanine Nucleotide Exchange Factor antibodyAnti-P115-Rhogef antibodyAnti-P115rhogef antibodyAnti-Sub1.5 antibodyAnti-ARHGEF1 antibody |
Information sourced from Uniprot.org