Anti-ARHGDIA antibody (1-204) (STJ22675)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ARHGDIA (1-204) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ARHGDIA |
| Gene ID | 396 |
| Uniprot ID | GDIR1_HUMAN |
| Immunogen Region | 1-204 |
| Immunogen Sequence | MAEQEPTAEQLAQIAAENEE DEHSVNYKPPAQKSIQEIQE LDKDDESLRKYKEALLGRVA VSADPNVPNVVVTGLTLVCS SAPGPLELDLTGDLESFKKQ SFVLKEGVEYRIKISFRVNR EIVSGMKYIQHTYRKGVKID KTDYMVGSYGPRAEEYEFLT PVEEAPKGMLARGSYSIKSR FTDDDKTDHLSWEWNLTIKK DWKD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human RhoGDI (NP_001172006.1). |
Additional Info
| Function | Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1. |
| Protein Name | Rho Gdp-Dissociation Inhibitor 1Rho Gdi 1Rho-Gdi Alpha |
| Database Links | Reactome: R-HSA-193634Reactome: R-HSA-209563Reactome: R-HSA-8980692Reactome: R-HSA-9013106Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013407Reactome: R-HSA-9013408 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Rho Gdp-Dissociation Inhibitor 1 antibodyAnti-Rho Gdi 1 antibodyAnti-Rho-Gdi Alpha antibodyAnti-ARHGDIA antibodyAnti-GDIA1 antibody |
Information sourced from Uniprot.org