Western Blot Antibodies | St John's Laboratory

Western blot analysis of CREB at dilution of 1:1000. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: NIH3T3 cell lysate Lane 4: mouse spleen tissue lysate Lane 5: mouse lung tissue lysate Lane 6: mouse brain tissue lysate Lane 7: Rat spleen tissue lysate Lane 8: Rat lung tissue lysate Lane 9: Rat brain tissue lysate

Anti-CREB antibody [SM0516] (STJA0010516)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£233.50

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Anti-CREB antibody [SRM13020] (STJ11107750)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£0.00

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Anti-CREB-1 antibody [PT0516R] (STJA0039481)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of various lysates using CREB1 Rabbit pAb (STJ11106047) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CREB1 antibody (1-120 aa) (STJ11106047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 300 Mu g extracts of LO2 cells using 3 Mu g CREB1 antibody (STJ113894). Western blot was performed from the immunoprecipitate using CREB1 antibody (STJ113894) at a dilution of 1:1000.

Anti-CREB1 antibody (1-341) (STJ113894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using CREB-1 Polyclonal Antibody diluted at 1：1000

Anti-CREB1 antibody (100-149 aa) (STJ92462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 1) A431, 2) 3T3 using CREB-1 monoclonal antibody.

Anti-CREB1 antibody (100-200 aa) [4B4] (STJ97445)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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STJ73024 (0. 1µg/ml) staining of Jurkat (A) and NIH3T3 (B) nuclear lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CREB1 antibody (96-109) (STJ73024)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Dog/Pig/Cow

£312.50

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Anti-CREB1 antibody (N-Term) (STJA0005350)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Chicken/Human/Mouse/Rat

£319.50

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Immunofluorescence analysis of PC-3 cells using CREB3 Rabbit polyclonal antibody (STJ28650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CREB3 antibody (1-230) (STJ28650)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 3T3 cells using CREB3 Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-CREB3 antibody (151-200 aa) (STJ98634)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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STJ73025 (0. 3µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CREB3 antibody (76-87) (STJ73025)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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Anti-CREB3L1 antibody (STJ192062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates, using CREB3L2 Rabbit polyclonal antibody (STJ112572) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CREB3L2 antibody (1-247) (STJ112572)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of various cells using CREB3L2 Polyclonal Antibody

Anti-CREB3L2 antibody (269-318 aa) (STJ92469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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STJ73026 (1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CREB3L2 antibody (N-Term) (STJ73026)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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Anti-CREB3L2 antibody [SRM13486] (STJ11108216)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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STJ72109 (1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CREB3L2/BBF2H7 antibody (Internal) (STJ72109)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Liu, Xiaomei, et al. "Maternal protein restriction induces alterations in hepatic unfolded protein response-related molecules in adult rat offspring. " Frontiers in endocrinology 9 (2018).

Anti-CREB3L3 antibody (1-100 aa) (STJ191913)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CREB3L4 antibody (200-280 aa) (STJ190423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using CREB5 antibody (STJ116842) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CREB5 antibody (210-475) (STJ116842)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CREB5 antibody (415-465 aa) (STJ196120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using CBP Polyclonal Antibody diluted at 1：1000

Anti-CREBBP antibody (1501-1550 aa) (STJ92058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Whole cell lysates were separated by 6% SDS-PAGE, and the membrane was blotted with anti-CREBBP (PT0619) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: MCF7Predicted band size: 266kDaObserved band size: 266kDa

Anti-CREBBP antibody (900-1000) [PT0619] (STJA0006228)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£49.00 - £261.00

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Anti-CREBBP antibody [PT0526R] (STJA0039426)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Anti-CREBBP antibody [SRM14923] (STJ11109653)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-CREBL2 antibody (STJA0008964)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CREBRF antibody (74-124 aa) (STJ193940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using CREBZF Rabbit polyclonal antibody (STJ26330) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.

Anti-CREBZF antibody (1-354) (STJ26330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of HepG2 cells, using CREBZF Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CREBZF antibody (221-270 aa) (STJ96308)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of K-562, using CREG1 antibody (STJ118521) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CREG1 antibody (110-190) (STJ118521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CREG1 antibody (STJ192205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-CREG2 antibody (30-110 aa) (STJ192447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-CRELD1 antibody (350-430 aa) (STJ192523)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of L929 cells using CRELD1 Rabbit polyclonal antibody (STJ117380) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CRELD1 antibody (50-150) (STJ117380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-CRELD2 antibody (257-307 aa) (STJ193492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using CREM antibody (STJ27591) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CREM antibody (1-100) (STJ27591)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-CREM antibody (81-130 aa) (STJ92471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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STJ70609 staining (1µg/ml) of Human Heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Anti-CREM antibody (C-Term) (STJ70609)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Anti-CREPT antibody [3E10#] (STJA0019707)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IHC/WB

Reactivity :

Human

£209.50 - £1,333.50

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Western blot analysis of extracts of various cell lines, using CRH antibody (STJ23221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CRH antibody (25-194) (STJ23221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of HeLa cells using CRHBP antibody (STJ28651). Blue: DAPI for nuclear staining.

Anti-CRHBP antibody (25-322) (STJ28651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CRHBP antibody (70-150 aa) (STJ192426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using CRHR1 Rabbit pAb (STJ110707) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-CRHR1 antibody (24-120) (STJ110707)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of PC12 cells using CRF-RI Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CRHR1 antibody (71-120 aa) (STJ96855)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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STJ71309 (1µg/ml) staining of Human Colon (A) , Cerebellum (B) , Rat Brain (C) and Mouse spinal Cord (D) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CRHR1/CRF-R antibody (107-117) (STJ71309)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat

£312.50

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STJ71060 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-CRHR1/CRF-R antibody (250-263) (STJ71060)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow/Sheep

£312.50

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Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-CRHR2 (PTR1319) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Neuro-2aPredicted band size: 47kDaObserved band size: 47kDa

Anti-CRHR2 antibody (50-150) [PTR1205] (STJA0006274)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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