| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CHMP6 (111-200) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CHMP6 |
| Gene ID: |
79643 |
| Uniprot ID: |
CHMP6_HUMAN |
| Immunogen Region: |
111-200 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 111-200 of human CHMP6 (NP_078867.2). |
| Immunogen Sequence: |
LNKMHQVMSIEEVERILDET QEAVEYQRQIDELLAGSFTQ EDEDAILEELSAITQEQIEL PEVPSEPLPEKIPENVPVKA RPRQAELVAA |
| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | ISGylated in a CHMP5-dependent manner. Isgylation weakens its interaction with VPS4A. |
| Function | Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes. |
| Protein Name | Charged Multivesicular Body Protein 6Chromatin-Modifying Protein 6Vacuolar Protein Sorting-Associated Protein 20Vps20Hvps20 |
| Database Links | Reactome: R-HSA-162588Reactome: R-HSA-1632852Reactome: R-HSA-5620971Reactome: R-HSA-917729Reactome: R-HSA-9610379Reactome: R-HSA-9615710Reactome: R-HSA-9668328Reactome: R-HSA-9679504Reactome: R-HSA-9694676 |
| Cellular Localisation | Endomembrane SystemEndosome MembraneLipid-AnchorLate Endosome MembraneMembraneLocalizes To Endosomal Membranes |
| Alternative Antibody Names | Anti-Charged Multivesicular Body Protein 6 antibodyAnti-Chromatin-Modifying Protein 6 antibodyAnti-Vacuolar Protein Sorting-Associated Protein 20 antibodyAnti-Vps20 antibodyAnti-Hvps20 antibodyAnti-CHMP6 antibodyAnti-VPS20 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
