• Western blot analysis of HeLa cells using CHP Polyclonal Antibody

Anti-CHP1 antibody (Internal) (STJ92279)

SKU:
STJ92279

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Calcineurin B homologous protein 1 (Internal) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHP1
Gene ID: 11261
Uniprot ID: CHP1_HUMAN
Immunogen Region: Internal
Specificity: CHP Polyclonal Antibody detects endogenous levels of CHP protein.
Immunogen: Synthesized peptide derived from the Internal region of human CHP.
Post Translational Modifications Phosphorylated.decreased phosphorylation is associated with an increase in SLC9A1/NHE1 Na(+)/H(+) exchange activity. Phosphorylation occurs in serum-dependent manner. The phosphorylation state may regulate the binding to SLC9A1/NHE1. Both N-myristoylation and calcium-mediated conformational changes are essential for its function in exocytic traffic. N-myristoylation is required for its association with microtubules and interaction with GAPDH, but not for the constitutive association to membranes.
Function Calcium-binding protein involved in different processes such as regulation of vesicular trafficking, plasma membrane Na(+)/H(+) exchanger and gene transcription. Involved in the constitutive exocytic membrane traffic. Mediates the association between microtubules and membrane-bound organelles of the endoplasmic reticulum and Golgi apparatus and is also required for the targeting and fusion of transcytotic vesicles (TCV) with the plasma membrane. Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Affects the pH sensitivity of SLC9A1/NHE1 by increasing its sensitivity at acidic pH. Required for the stabilization and localization of SLC9A1/NHE1 at the plasma membrane. Inhibits serum- and GTPase-stimulated Na(+)/H(+) exchange. Plays a role as an inhibitor of ribosomal RNA transcription by repressing the nucleolar UBF1 transcriptional activity. May sequester UBF1 in the nucleoplasm and limit its translocation to the nucleolus. Associates to the ribosomal gene promoter. Acts as a negative regulator of the calcineurin/NFAT signaling pathway. Inhibits NFAT nuclear translocation and transcriptional activity by suppressing the calcium-dependent calcineurin phosphatase activity. Also negatively regulates the kinase activity of the apoptosis-induced kinase STK17B. Inhibits both STK17B auto- and substrate-phosphorylations in a calcium-dependent manner.
Protein Name Calcineurin B Homologous Protein 1
Calcineurin B-Like Protein
Calcium-Binding Protein Chp
Calcium-Binding Protein P22
Ef-Hand Calcium-Binding Domain-Containing Protein P22
Database Links Reactome: R-HSA-2160916
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Endomembrane System
Endoplasmic Reticulum-Golgi Intermediate Compartment
Endoplasmic Reticulum
Cell Membrane
Membrane
Lipid-Anchor
Localizes In Cytoplasmic Compartments In Dividing Cells
Localizes In The Nucleus In Quiescent Cells
Exported From The Nucleus To The Cytoplasm Through A Nuclear Export Signal (Nes) And Crm1-Dependent Pathway
May Shuttle Between Nucleus And Cytoplasm
Localizes With The Microtubule-Organizing Center (Mtoc) And Extends Toward The Periphery Along Microtubules
Associates With Membranes Of The Early Secretory Pathway In A Gapdh-Independent
N-Myristoylation- And Calcium-Dependent Manner
Colocalizes With The Mitotic Spindle Microtubules
Colocalizes With Gapdh Along Microtubules
Colocalizes With Slc9a1 At The Reticulum Endoplasmic And Plasma Membrane
Colocalizes With Stk17b At The Plasma Membrane
Alternative Antibody Names Anti-Calcineurin B Homologous Protein 1 antibody
Anti-Calcineurin B-Like Protein antibody
Anti-Calcium-Binding Protein Chp antibody
Anti-Calcium-Binding Protein P22 antibody
Anti-Ef-Hand Calcium-Binding Domain-Containing Protein P22 antibody
Anti-CHP1 antibody
Anti-CHP antibody

Information sourced from Uniprot.org

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