Anti-CHMP6 antibody (87-137 aa) (STJ195445)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human CHMP6 at the amino acid range 87-137
General Information
| Short Description | Rabbit polyclonal anti-Charged multivesicular body protein 6 (87-137 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CHMP6 |
| Gene ID | 79643 |
| Uniprot ID | CHMP6_HUMAN |
| Immunogen | Synthesized peptide derived from the human CHMP6 at the amino acid range 87-137 |
| Immunogen Region | 87-137 aa |
| Specificity | This antibody detects endogenous levels of CHMP6 at Human/Mouse |
Additional Info
| Post Translational Modifications | ISGylated in a CHMP5-dependent manner. Isgylation weakens its interaction with VPS4A. |
| Function | Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes. |
| Protein Name | Charged Multivesicular Body Protein 6Chromatin-Modifying Protein 6Vacuolar Protein Sorting-Associated Protein 20Vps20Hvps20 |
| Database Links | Reactome: R-HSA-162588Reactome: R-HSA-1632852Reactome: R-HSA-5620971Reactome: R-HSA-917729Reactome: R-HSA-9610379Reactome: R-HSA-9615710Reactome: R-HSA-9668328Reactome: R-HSA-9679504Reactome: R-HSA-9694676 |
| Cellular Localisation | Endomembrane SystemEndosome MembraneLipid-AnchorLate Endosome MembraneMembraneLocalizes To Endosomal Membranes |
| Alternative Antibody Names | Anti-Charged Multivesicular Body Protein 6 antibodyAnti-Chromatin-Modifying Protein 6 antibodyAnti-Vacuolar Protein Sorting-Associated Protein 20 antibodyAnti-Vps20 antibodyAnti-Hvps20 antibodyAnti-CHMP6 antibodyAnti-VPS20 antibody |
Information sourced from Uniprot.org