Western Blot Antibodies | St John's Laboratory

Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-CD8 a (ABT455) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Daudi Lane 2: Raji Lane 3: MOLT-4Predicted band size: 26kDaObserved band size: 24kDa

Anti-CD8A antibody (100-235 aa) [ABT304] (STJ197218)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of extracts of various cell lines, using CD8A rabbit monoclonal antibody (STJ11101251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CD8A antibody (136-235) [S1MR] (STJ11101251)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-CD8A antibody (22-182 aa) [SRM] (STJ11106940)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human tonsil using CD8A Rabbit polyclonal antibody (STJ114922) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD8A antibody (22-235) (STJ114922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various lysates, using CD8A Rabbit polyclonal antibody (STJ11104719) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD8A antibody (STJ11104719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Anti-CD8A antibody (STJA0010229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Cd8a antibody [53-6.7] (STJA0001670)

Clonality :

Monoclonal

Host :

Rat

Applications :

Block/Depletion/FC/WB/IF/IP

Reactivity :

Mouse

£93.50 - £188.50

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Anti-Cd8a antibody [53-6.7] (STJA0001671)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/WB/IF/IP

Reactivity :

Mouse

£93.50 - £114.50

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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD8B using CD8B Rabbit polyclonal antibody (STJ23016) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD8B antibody (22-170) (STJ23016)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CD8B antibody (STJ192945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using CD9 Polyclonal Antibody diluted at 1：1000

Anti-CD9 antibody (101-150 aa) (STJ92147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of CD9 in paraffin-embedded human smooth muscle using CD9 Rabbit polyclonal antibody (STJ23017) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CD9 antibody (112-195) (STJ23017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CD9 antibody (115-195 aa) (STJ140294)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Reacts against human, rat, mouse, canine and monkey proteins.

£135.50 - £394.50

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Immunoprecipitation analysis of 300 Mu g extracts from THP-1 cells using 3 Mu g CD9 Rabbit monoclonal antibody (STJ11101565). Western blot was performed from the immunoprecipitate using CD9 Rabbit monoclonal antibody (STJ11101565) at a dilution of 1:1000.

Anti-CD9 antibody (129-228) [S5MR] (STJ11101565)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Anti-CD9 antibody (N-Term) (STJA0005794)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£455.50

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Anti-CD9 antibody [BU16] (STJ16101389)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/FC/IF

Reactivity :

Human

£741.50

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Anti-CD9 antibody [MM2/57] (STJ16100822)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC/IHC-F

Reactivity :

Human/Llama/Mink/Mustelid/Ferret/Dog/Horse/Bovine/Cat/Rhesus/Pig/Rabbit

£741.50

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Western blot analysis of Mouse spleen, using CD90. 1 Rabbit polyclonal antibody (STJ11105183) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CD90.1 antibody (20-120 aa) (STJ11105183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human liver using CD90/Thy1 Rabbit monoclonal antibody (STJ11102747) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD90/Thy1 antibody (1-100) [S7MR] (STJ11102747)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£129.50 - £574.50

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STJ71954 (0. 1µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CD90/Thy1 antibody (Internal) (STJ71954)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Dog/Pig

£312.50

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Anti-CD91 antibody [A2Mr alpha-2] (STJ16100538)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/FC/IHC-F/ELISA

Reactivity :

Human

£646.50

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Western blot analysis of KB cells using CD93 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD93 antibody (191-240 aa) (STJ97292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using CD96 Rabbit polyclonal antibody (STJ11103987) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CD96 antibody (270-370) (STJ11103987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 293-UV cells using CD96 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-CD96 antibody (291-340 aa) (STJ97293)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of Mouse lung, using CD97 Rabbit polyclonal antibody (STJ11105186) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD97 antibody (24-265 aa) (STJ11105186)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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STJ72921 (0. 3µg/ml) staining of Mouse Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Cd97-Mouse antibody (C-Term) (STJ72921)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Mouse

£312.50

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Western blot analysis of various lysates, using CD98 antibody (STJ11104992) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD98 antibody (105-526 aa) (STJ11104992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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LN18 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD99 (ABT156) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: LN18Predicted band size: 19kDaObserved band size: 28kDa

Anti-CD99 antibody (1-100 aa) [ABT156] (STJ197127)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of various lysates using CD99 Rabbit mAb (STJ11105761) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-CD99 antibody (1-100) [S5761RM] (STJ11105761)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of various lysates, using CD99 Rabbit polyclonal antibody (STJ11105426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) :CHOExposure time: 90s.

Anti-CD99 antibody (23-124) (STJ11105426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of Jurkat cells using CD99 Rabbit polyclonal antibody (STJ23019) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD99 antibody (23-185) (STJ23019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemistry of baboon small intestine with Anti-CD99 primary antibody (STJ500489) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 20X.

Anti-CD99 antibody (50-100) (STJ500489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£427.50

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Anti-CD99 antibody (50-100) {Biotin} (STJ500491)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

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Anti-CD99 antibody (50-100) {FITC} (STJ500492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

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Anti-CD99 antibody (97-147) (STJ500490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey

£427.50

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Anti-CD99 antibody (97-147) {Biotin} (STJ500493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey

£528.50

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Anti-CD99 antibody (97-147) {FITC} (STJ500494)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey

£528.50

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Anti-CD99 antibody (Extracellular region) [M026] (STJA0005329)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

£390.50

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Anti-CD99 antibody (Extracellular region) [M030] (STJA0005330)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-CD99 antibody [EBS-CD-044] (STJ16102589)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human/Rat

£307.50 - £1,449.50

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Anti-CD99 antibody [MEM-131] (STJ16100090)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB/IHC-P

Reactivity :

Human

£431.50

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CD99L2 antibody (204-254 aa) (STJ195459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using CD99L2 Rabbit polyclonal antibody (STJ118352) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD99L2 antibody (26-185) (STJ118352)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from Mouse kidney using CDA Rabbit polyclonal antibody (STJ11104671) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:10s.

Anti-CDA antibody (1-146 aa) (STJ11104671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and CDA knockout (KO) HeLa cells, using [KO Validated] CDA Rabbit polyclonal antibody (STJ11100721) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CDA antibody (1-146) (STJ11100721)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Anti-CDA antibody (1-146) (STJ11100722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of various lysates, using CDA antibody (STJ115894) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CDA antibody (1-146) (STJ115894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of mouse kidney, using CDA antibody (STJ23020) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDA antibody (1-146) (STJ23020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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