Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (STJ24482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MAPK1 antibody (200-300) (STJ24482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

Choose Options

Confocal imaging of HeLa cells using ERK1/2 Rabbit monoclonal antibody (STJ11102154, dilution 1:50) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-MAPK1/MAPK3 antibody (180-360) [S4MR] (STJ11102154)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€172.20 - €742.20

Choose Options

Western blot analysis of extracts of various cell lines, using ERK1/ERK2 antibody (STJ119100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MAPK1/MAPK3 antibody (200-300) (STJ119100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

Choose Options

Confocal immunofluorescence analysis of Hela cells using MAPK1/MAPK3 Rabbit polyclonal antibody (STJ119147) at dilution of 1:200. Blue: DAPI for nuclear staining.

Anti-MAPK1/MAPK3 antibody (200-300) (STJ119147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

Choose Options

Western blot analysis of various lysates, using ERK1 antibody (STJ11101698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAPK3 antibody (1-100) [S8MR] (STJ11101698)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

Choose Options

Immunohistochemistry analysis of ERK1 in paraffin-embedded mouse heart using [KD Validated] ERK1 Rabbit polyclonal antibody (STJ24487) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAPK3 antibody (1-378) (STJ24487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

Choose Options

Western blot analysis of various cells using ERK 1 Polyclonal Antibody diluted at 1：500

Anti-MAPK3 antibody (161-210 aa) (STJ92987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

Choose Options

Immunofluorescence analysis of PC-12 using ERK1 Rabbit monoclonal antibody (STJ11104626) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAPK3 antibody (200-300) [S4626RM] (STJ11104626)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Rat

€151.80 - €665.40

Choose Options

Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-MAPK3 antibody (STJ197394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

Choose Options

STJ70702 (0. 1µg/ml) staining of Human Cerebellum (A) and Frontal Cortex (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-MAPK3/ERK1 antibody (N-Term) (STJ70702)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human

€375.00

Choose Options

Immunofluorescence analysis of L929 cells using MAPK6 Rabbit polyclonal antibody (STJ117499) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAPK6 antibody (372-721) (STJ117499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

Choose Options

Western blot analysis of Hela Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-MAPK6 antibody (461-510 aa) (STJ92993)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

Choose Options

Western blot analysis of various cells using MARK1/2/3/4 Polyclonal Antibody

Anti-MARK1/2/3/4 antibody (181-230 aa) (STJ94015)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

Choose Options

Western blot analysis of various cells using MARK2 Polyclonal Antibody

Anti-MARK2 antibody (10-59 aa) (STJ94016)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

Choose Options

Immunofluorescence analysis of L929 cells using MARK2 Rabbit polyclonal antibody (STJ119118) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MARK2 antibody (350-455) (STJ119118)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

Choose Options

Western blot analysis of various cells using C-TAK1 Polyclonal Antibody diluted at 1：500

Anti-MARK3 antibody (1-50 aa) (STJ92512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

Choose Options

MARK3 antibody western blot K562 cells STJA0037033

Anti-MARK3 antibody [R09-4E1] (STJA0037033)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP/FC

Reactivity :

Mouse/Human

€160.20 - €337.80

Choose Options

Immunofluorescence analysis of A549 cells, using MARK4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MARK4 antibody (461-510 aa) (STJ94018)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Mouse

€58.80 - €313.20

Choose Options

Immunofluorescence analysis of Hela cell. 1, MEK-1/2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CYCS monoclonal antibody (4B10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-MEK-1/2 antibody (189-238 aa) (STJ94080)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

Choose Options

MEK1 antibody western blot A431 cells STJA0037554

Anti-MEK1 antibody (STJA0037554)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

Choose Options

MEK1 antibody immunocytochemistry HeLa cells STJA0035018

Anti-MEK1 antibody [R01-3C2] (STJA0035018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

€160.20 - €337.80

Choose Options

MEK1D67N antibody western blot recombinant protein STJA0033558

Anti-MEK1-D67N Mutant antibody (STJA0033558)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/ELISA

Reactivity :

Vertebrates

€419.40 - €623.40

Choose Options

MEK1F53L antibody western blot recombinant protein STJA0033634

Anti-MEK1-F53L Mutant antibody (STJA0033634)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/ELISA

Reactivity :

Vertebrates

€419.40 - €623.40

Choose Options

MEK1 I111S antibody western blot recombinant protein STJA0033635

Anti-MEK1-I111S Mutant antibody (STJA0033635)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/ELISA

Reactivity :

Vertebrates

€419.40 - €623.40

Choose Options

MEK1N382H antibody western blot recombinant protein STJA0033638

Anti-MEK1-N382H Mutant antibody (STJA0033638)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/ELISA

Reactivity :

Vertebrates

€419.40 - €623.40

Choose Options

MEK1 Q56P antibody western blot recombinant protein STJA0033633

Anti-MEK1-Q56P Mutant antibody (STJA0033633)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ICC/IF/ELISA

Reactivity :

Vertebrates

€419.40 - €623.40

Choose Options

MEK1/2 antibody western blot K562 C6 3T3 HeLa STJA0034557

Anti-MEK1/2 antibody [R08-6A7] (STJA0034557)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

Choose Options

MEK2 antibody western blot Jurkat cells STJA0036142

Anti-MEK2 antibody [R01-9J3] (STJA0036142)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse

€160.20 - €337.80

Choose Options

MEK2 antibody western blot mouse brain lung STJA0035265

Anti-MEK2 antibody [R05-2G7] (STJA0035265)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse

€160.20 - €337.80

Choose Options

Anti-MEK2/MAP2K2 antibody [2H4] (STJ16103497)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

€895.80

Choose Options

MEK3 antibody western blot C6 3T3 HeLa STJA0034558

Anti-MEK3 antibody [R06-1F1] (STJA0034558)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

Choose Options

Anti-MEK3 antibody [SRM13596] (STJ11108326)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

Choose Options

MEK3/MEK6 antibody immunocytochemistry HCT116 cells STJA0035414

Anti-MEK3/MEK6 antibody [R04-2I0] (STJA0035414)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

€160.20 - €337.80

Choose Options

Anti-MEK4/MKK4 antibody [R06-2V-6] (STJA0033154)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human

€257.40 - €370.20

Choose Options

MEK5 antibody western blot HeLa lysates STJA0036147

Anti-MEK5 antibody [R05-6E6] (STJA0036147)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

€160.20 - €337.80

Choose Options

MEK7 antibody western blot HeLa lysates STJA0036148

Anti-MEK7 antibody [R08-3F5] (STJA0036148)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

Choose Options

Western blot analysis of HT29+PMA cells using Phospho-Cdk5 (Y15) Polyclonal Antibody

Anti-Phospho-CDK5-Tyr15 antibody (1-50 aa) (STJ90540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

Choose Options

Immunofluorescence analysis of mouse-liver tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-ERK 1/2-Thr202 antibody (169-218 aa) (STJ90260)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

Choose Options

Immunofluorescence analysis of rat-lung tissue. 1, ERK 1/2 (phospho Thr202/Y204) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-ERK 1/2-Thr202/Y204 antibody (STJ91357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Fish

€58.80 - €313.20

Choose Options

Immunofluorescence analysis of rat-spleen tissue. 1, ERK 1/2 (phospho Tyr204) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-ERK 1/2-Tyr204 antibody (170-219 aa) (STJ90261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

Choose Options

Immunofluorescence analysis of Hela cell. 1, ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-tubulin monoclonal antibody (M7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-ERK-Tyr222/205 antibody (STJ90657)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

Choose Options

Western Blot (WB): The picture was kindly provided by our customer. The primary antibody was diluted at 1:2000. The STJ97738

Anti-Phospho-ERK-Y222/205 antibody (140-220 aa) [4G3] (STJ97738)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

Choose Options

Phospho-ERK1/2 antibody western blot NIH/3T3 lysates STJA0035674

Anti-Phospho-ERK1/2-Thr202/Thr185 antibody [R49-1Y-4] (STJA0035674)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

Choose Options

phospho-ERK1/2 antibody western blot A431 lysates EGF STJA0035749

Anti-Phospho-ERK1/2-Thr202/Tyr204+Thr185/Tyr187 antibody [R08-8S3] (STJA0035749)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

€160.20 - €337.80

Choose Options

Western blot analysis of LOVO+UV cells using Phospho-Fyn (Y530) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-FYN-Tyr530 antibody (488-537 aa) (STJ90792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

Choose Options

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-MAP2-Thr1616 antibody (STJ193213)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

Choose Options

Western blot analysis of HepG2-UV using p-MEK-1 (S298) antibody. Antibody was diluted at 1:500. STJ90489

Anti-Phospho-MAP2K1-Ser298 antibody (264-313 aa) (STJ90489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

Choose Options

Western blot analysis of lysates from K562 cells, using MEK1 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAP2K1-Thr286 antibody (252-301 aa) (STJ90854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

Choose Options