• Immunofluorescence analysis of Human-liver-cancer tissue. 1, ABCB5 monoclonal antibody (11A2) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of 1) Hela, 2) 293T, diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, ABCB5 monoclonal antibody (11A2) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-ABCB5 antibody [11A2] (STJ96976)

SKU:
STJ96976

Current Stock:
Host: Mouse
Applications: WB, IHC-P, IF, ICC
Reactivity: Human
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Mouse monoclonal antibody anti-Atp-Binding Cassette Sub-Family B Member 5 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: 11A2
Conjugation: Unconjugated
Isotype: IgG1
Formulation: PBS, pH 7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was isolated from ascitic fluid by immunoaffinity chromatography using antigens coupled to agarose beads.
Dilution Range: WB 1:2000
IF 1:200
IHC 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABCB5
Gene ID: 340273
Uniprot ID: ABCB5_HUMAN
Specificity: ABCB5 monoclonal antibody (Atp-Binding Cassette Sub-Family B Member 5) binds to endogenous Atp-Binding Cassette Sub-Family B Member 5.
Immunogen: Synthetic peptide of ABCB5
Function Energy-dependent efflux transporter responsible for decreased drug accumulation in multidrug-resistant cells. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.
Protein Name Atp-Binding Cassette Sub-Family B Member 5
Abcb5 P-Gp
P-Glycoprotein Abcb5
Database Links Reactome: R-HSA-382556
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Atp-Binding Cassette Sub-Family B Member 5 antibody
Anti-Abcb5 P-Gp antibody
Anti-P-Glycoprotein Abcb5 antibody
Anti-ABCB5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance