• STJ71461 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71461 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71461 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-AADACL1 antibody (C-Term) (STJ71461)

SKU:
STJ71461-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human/Mouse/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-AADACL1 (C-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the Endoplasmic reticulum of A431 and U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:1000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NCEH1
Gene ID: 57552
Uniprot ID: NCEH1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_065843.3; NP_001139748.2; NP_001139749.1
Immunogen Sequence: RTRNSYIKWLDQN
Post Translational Modifications N-glycosylated.
Function Hydrolyzes 2-acetyl monoalkylglycerol ether (1-O-alkyl-2-acetyl-sn-glycerol), the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for the hydrolysis of cholesterol esters (such as cholesteryl (9Z-octadecenoate)) in macrophages. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.
Protein Name Neutral Cholesterol Ester Hydrolase 1
Nceh
Acetylalkylglycerol Acetylhydrolase
2-Acetyl Mage Hydrolase
Arylacetamide Deacetylase-Like 1
Database Links Reactome: R-HSA-8964038
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Microsome
Alternative Antibody Names Anti-Neutral Cholesterol Ester Hydrolase 1 antibody
Anti-Nceh antibody
Anti-Acetylalkylglycerol Acetylhydrolase antibody
Anti-2-Acetyl Mage Hydrolase antibody
Anti-Arylacetamide Deacetylase-Like 1 antibody
Anti-NCEH1 antibody
Anti-AADACL1 antibody
Anti-KIAA1363 antibody

Information sourced from Uniprot.org

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