• Western blot analysis of extracts of HepG2 cells, using ABCA1 antibody (STJ29308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of extracts of various cell lines, using ABCA1 antibody (STJ29308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of C6 cells using ABCA1 rabbit polyclonal antibody (STJ29308) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ABCA1 rabbit polyclonal antibody (STJ29308) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ABCA1 antibody (1870-2261) (STJ29308)

SKU:
STJ29308

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Host: Rabbit
Applications: IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ABCA1 (1870-2261) is suitable for use in Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABCA1
Gene ID: 19
Uniprot ID: ABCA1_HUMAN
Immunogen Region: 1870-2261
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1870-2261 of human ABCA1 (NP_005493.2).
Immunogen Sequence: IQYRFFIRPRPVNAKLSPLN DEDEDVRRERQRILDGGGQN DILEIKELTKIYRRKRKPAV DRICVGIPPGECFGLLGVNG AGKSSTFKMLTGDTTVTRGD AFLNKNSILSNIHEVHQNMG YCPQFDAITELLTGREHVEF FALLRGVPEKEVGKVGEWAI RKLGLVKYGEKYAGNYSGGN KRKLSTAMALIGGPPVVFLD EPTTGMDPKARRFLWNCALS VVKEGRSVVLTSHSMEECE
Tissue Specificity Widely expressed, but most abundant in macrophages.
Post Translational Modifications Phosphorylation on Ser-2054 regulates phospholipid efflux. Palmitoylated by ZDHHC8. Palmitoylation is essential for localization to the plasma membrane.
Function Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP. Thereby, participates in phospholipid transfer to apolipoproteins to form nascent high density lipoproteins/HDLs. Transports preferentially phosphatidylcholine over phosphatidylserine. May play a similar role in the efflux of intracellular cholesterol to apolipoproteins and the formation of nascent high density lipoproteins/HDLs. Translocates phospholipids from the outer face of the plasma membrane and forces it through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain.
Protein Name Phospholipid-Transporting Atpase Abca1
Atp-Binding Cassette Sub-Family A Member 1
Atp-Binding Cassette Transporter 1
Abc-1
Atp-Binding Cassette 1
Cholesterol Efflux Regulatory Protein
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-5682113
Reactome: R-HSA-8963896
Reactome: R-HSA-9029569
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Endosome
Alternative Antibody Names Anti-Phospholipid-Transporting Atpase Abca1 antibody
Anti-Atp-Binding Cassette Sub-Family A Member 1 antibody
Anti-Atp-Binding Cassette Transporter 1 antibody
Anti-Abc-1 antibody
Anti-Atp-Binding Cassette 1 antibody
Anti-Cholesterol Efflux Regulatory Protein antibody
Anti-ABCA1 antibody
Anti-ABC1 antibody
Anti-CERP antibody

Information sourced from Uniprot.org

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