Anti-AARS antibody (STJ11107772)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit polyclonal Alanine--Trna Ligase-Cytoplasmic antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB, 1:500-1:2000IHC-P, 1:50-1:200IF/ICC, 1:50-1:200ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AARS1 |
| Gene ID | 16 |
| Uniprot ID | SYAC_HUMAN |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Immunogen Sequence | Human |
Additional Info
| Function | Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. In presence of high levels of lactate, also acts as a protein lactyltransferase that mediates lactylation of lysine residues in target proteins, such as TEAD1, TP53/p53 and YAP1. Protein lactylation takes place in a two-step reaction: lactate is first activated by ATP to form lactate-AMP and then transferred to lysine residues of target proteins. Acts as an inhibitor of TP53/p53 activity by catalyzing lactylation of TP53/p53. Acts as a positive regulator of the Hippo pathway by mediating lactylation of TEAD1 and YAP1. |
| Protein Name | Alanine--Trna Ligase - CytoplasmicAlanyl-Trna SynthetaseAlarsProtein Lactyltransferase Aars1Renal Carcinoma Antigen Ny-Ren-42 |
| Database Links | Reactome: R-HSA-379716 |
| Cellular Localisation | CytoplasmNucleusTranslocates To The Nucleus In Response To Increased Levels Of LactateNuclear Translocation Is Dependent On Kpna4 |
| Alternative Antibody Names | Anti-Alanine--Trna Ligase - Cytoplasmic antibodyAnti-Alanyl-Trna Synthetase antibodyAnti-Alars antibodyAnti-Protein Lactyltransferase Aars1 antibodyAnti-Renal Carcinoma Antigen Ny-Ren-42 antibodyAnti-AARS1 antibodyAnti-AARS antibody |
Information sourced from Uniprot.org