Flow Cytometry Antibodies | St John's Laboratory

Western blot analysis of lysates from control 293T and SARS-CoV-2 Spike S1-293T trasfected cells, using SARS-CoV-2 Spike S1 mAb, BSA and glycerol free (STJ11101211) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-2019-nCoV antibody [S1MR] (STJ11101211)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

SARS-CoV-2

£126.50 - £554.50

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Western blot analysis of extracts of various cell lines, using MMP14/MMP14/MT1-MMP rabbit monoclonal antibody (STJ11101224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MMP14 antibody (100-200) [S4MR] (STJ11101224)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/FC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using CD8A rabbit monoclonal antibody (STJ11101251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CD8A antibody (136-235) [S1MR] (STJ11101251)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Flow cytometry:1X10^6 Raji cells (negative control, left) and Jurkat cells (right) were intracellularly-stained with CD3H Rabbit monoclonal antibody (STJ11101284, 10 Mu g/mL, orange line) or Rabbit IgG isotype control ( 10 Mu g/mL, blue line) , followed by FITC conjugated goat anti-rabbit polyclonal antibody (1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).

Anti-CD247 antibody (65-164) [S4MR] (STJ11101284)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/FC/ELISA

Reactivity :

Human

£126.50 - £554.50

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Immunohistochemistry analysis of CD11b/ITGAM in paraffin-embedded human lung cancer using CD11b/ITGAM Rabbit monoclonal antibody (STJ11101548) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ITGAM antibody (1053-1152) [S8MR] (STJ11101548)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/FC/ELISA

Reactivity :

Human

£126.50 - £554.50

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Western blot analysis of extracts of various cell lines, using CD14 antibody (STJ11101549) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CD14 antibody (250-350) [S9MR] (STJ11101549)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using Integrin alpha V (ITGAV/CD51) (ITGAV/CD51) antibody (STJ11101608) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ITGAV antibody (949-1048) [S8MR] (STJ11101608)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/FC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using Integrin beta 3 (ITGB3/CD61) antibody (STJ11101610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ITGB3 antibody (689-788) [S0MR] (STJ11101610)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/FC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-MS4A1 antibody (100-200) [S5MR] (STJ11102185)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/FC/ELISA

Reactivity :

Human

£143.50 - £618.50

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Western blot analysis of lysates from Jurkat cells, using CD3D Rabbit mAb (STJ11102545) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-CD3D antibody (72-171) [S5MR] (STJ11102545)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of Jurkat cells, using CD3E antibody (STJ22996) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CD3E antibody (111-207) (STJ22996)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/FC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using CD25 antibody (STJ24183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-IL2RA antibody (153-167) (STJ24183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using Glypican 3 (GPC3) (GPC3) antibody (STJ26187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GPC3 antibody (537-554) (STJ26187)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of Rat spleen, using CD7 antibody (STJ29964) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CD7 antibody (26-180) (STJ29964)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Western blot analysis of lysates from HeLa cells, primary antibody was diluted at 1:1000, 4°C overnight STJ194828

Anti-SHOX2 antibody (37-87 aa) (STJ194828)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/FC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from PC-12 cells, primary antibody was diluted at 1:1000, 4°C overnight STJ195768

Anti-STON2 antibody (47-97 aa) (STJ195768)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/FC/ELISA/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, Histone H3 (phospho Ser10) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-Histone H3-Ser10 antibody (1-50 aa) (STJ90289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IHC-F/FC/ICC/IF/ELISA

Reactivity :

Human/Mouse/Rat/D.melanogaster

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-MAPK14-Thr180/Y182 antibody (151-200 aa) (STJ90498)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/FC/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Guinea Pig

£49.00 - £261.00

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Western blot analysis of various cells using AR-Beta 2 Polyclonal Antibody

Anti-ADRB2 antibody (321-370 aa) (STJ91726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from Jurkat cells, using BNIP2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-BNIP2 antibody (265-314 aa) (STJ91877)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/FC/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western Blot analysis of various cells using C1q-B Polyclonal Antibody diluted at 1:1000 STJ91928

Anti-C1QB antibody (161-210 aa) (STJ91928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/FC/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, Calregulin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-CALR antibody (21-70 aa) (STJ91985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/FC/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of K562 cells using CD272 Polyclonal Antibody

Anti-BTLA antibody (101-150 aa) (STJ92108)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Frizzled-7 Polyclonal Antibody diluted at 1：2000

Anti-FZD7 antibody (45-94 aa) (STJ93151)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/FC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-IL4 antibody (C-Term) (STJ93700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of mouse brain cells using CD19 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-CD19 antibody (191-240 aa) (STJ97275)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/FC/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western Blot analysis of HeLa cells using CD42d Polyclonal Antibody. Secondary antibody was diluted STJ97282

Anti-GP5 antibody (331-380 aa) (STJ97282)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using ABCG2 monoclonal antibody against NIH/3T3 (1) and Cos7 (2) cell lysate.

Anti-ABCG2 antibody [3G8] (STJ97809)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Monkey

£149.00 - £360.00

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Western blot analysis using ACTA2 monoclonal antibody against HeLa (1) , A431 (2) , Jurkat (3) , K562 (4) , HEK293 (5) , HepG2 (6) , NIH/3T3 (7) , PC-12 (8) and Cos7 (9) cell lysate.

Anti-ACTA2 antibody [4A4] (STJ97813)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£149.00 - £360.00

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Western blot analysis using AIF-M1 monoclonal antibody against NIH/3T3 (1) , Jurkat (2) , HeLa (3) , HepG2 (4) , MOLT4 (5) , C6 (6) , RAJI (7) , Cos7 (8) and PC-12 (9) cell lysate.

Anti-AIFM1 antibody [4E7] (STJ97819)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£149.00 - £360.00

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Western blot analysis using AMPK Alpha 1 monoclonal antibody against Jurkat (1) , HeLa (2) , HepG2 (3) , MCF-7 (4) , Cos7 (5) , NIH/3T3 (6) , K562 (7) , HEK293 (8) , and PC-12 (9) cell lysate.

Anti-PRKAA1 antibody [2B7] (STJ97826)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£149.00 - £360.00

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Western blot analysis using AP-1 monoclonal antibody against NIH/3T3 (1) and Cos7 (2) cell lysate.

Anti-JUN antibody [5B1] (STJ97829)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Monkey

£149.00 - £360.00

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Western blot analysis using ApoE monoclonal antibody against HEK293 (1) and ApoE (AA: 20-267) -hIgGFc transfected HEK293 (2) cell lysate.

Anti-APOE antibody [1H4] (STJ97839)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis using ARK-1 monoclonal antibody against HEK293 (1) , Sw620 (2) , MCF-7 (3) , Jurkat (4) , HeLa (5) , HepG2 (6) , Cos7 (7) and PC-12 (8) cell lysate.

Anti-AURKA antibody [1F8] (STJ97849)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human/Rat/Monkey

£149.00 - £360.00

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Western blot analysis using ASK 1 monoclonal antibody against recombinant protein.

Anti-MAP3K5 antibody [2E4] (STJ97852)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis using Ataxin-1 monoclonal antibody against HEK293 (1) and ATXN1-hIgGFc transfected HEK293 (2) cell lysate.

Anti-ATXN1 antibody [2F5] (STJ97853)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis using Bcl-10 monoclonal antibody against NIH/3T3 (1) , HeLa (2) , MCF-7 (3) and Jurkat (4) cell lysate.

Anti-BCL10 antibody [4F8] (STJ97859)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse

£149.00 - £360.00

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Western blot analysis using Beclin-1 monoclonal antibody against HeLa (1) , A431 (2) , MCF-7 (3) , RAJI (4) , Jurkat (5) and SKBR-3 (6) cell lysate.

Anti-BECN1 antibody [2A4] (STJ97862)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis using BID monoclonal antibody against HeLa (1) , A431 (2) , Jurkat (3) , A549 (4) , HepG2 (5) , and HEK293 (6) cell lysate.

Anti-BID antibody [3C5] (STJ97864)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis using Blk monoclonal antibody against HEK293 (1) and BLK-hIgGFc transfected HEK293 (2) cell lysate.

Anti-BLK antibody [1E6] (STJ97869)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis using BLNK monoclonal antibody against NIH/3T3 (1) and BCBL-1 (2) cell lysate.

Anti-BLNK antibody [5G9] (STJ97870)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse

£149.00 - £360.00

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Western blot analysis using BM28 monoclonal antibody against PC-12 (1) , Cos7 (2) , NIH/3T3 (3) , HepG2 (4) , HEK293 (5) , K562 (6) , Jurkat (7) , HeLa (8) and MCF-7 (9) cell lysate.

Anti-MCM2 antibody [2B3] (STJ97871)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£149.00 - £360.00

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Western blot analysis using Bmi-1 monoclonal antibody against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.

Anti-BMI1 antibody [3E3] (STJ97874)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis using C/EBP Beta monoclonal antibody against human C/EBP Beta (AA: 161-338) recombinant protein.

Anti-CEBPB antibody [3H9] (STJ97886)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis using Catenin-Beta monoclonal antibody against CTNNB1-hIgGFc transfected HEK293 cell lysate.

Anti-CTNNB1 antibody [4D5] (STJ97896)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis using Cbl monoclonal antibody against RAJI (1) , RAW264.7 (2) , K562 (3) , SKBR-3 (4) , 3T3-L1 (5) , THP-1 (6) and PC-12 (7) cell lysate.

Anti-CBL antibody [3B12] (STJ97898)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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Western blot analysis using CBX1 monoclonal antibody against HeLa (1) , COS7 (2) , NIH/3T3 (3) , A431 (4) , and C6 (5) cell lysate.

Anti-CBX1 antibody [5A3] (STJ97899)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human/Monkey

£149.00 - £360.00

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Western blot analysis using CD105 monoclonal antibody against HEK293 (1) and CD105-hIgGFc transfected HEK293 (2) cell lysate.

Anti-ENG antibody [3A9] (STJ97903)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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