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Anti-BLNK antibody [5G9] (STJ97870)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant fragment of human BLNK expressed in E. Coli.
General Information
| Short Description | Mouse monoclonal anti-B-cell linker protein for use in WB, IHC, IF, FC and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/FC/ELISA |
| Host | Mouse |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 5G9 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB 1:500-1:2000IHC 1:200-1:1000IF 1:200-1:1000FC 1:200-1:400ELISA 1:10000 |
| Formulation | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BLNK |
| Gene ID | 29760 |
| Uniprot ID | BLNK_HUMAN |
| Immunogen | Purified recombinant fragment of human BLNK expressed in E. Coli. |
| Specificity | BLNK Monoclonal Antibody detects endogenous levels of BLNK protein. |
Additional Info
| Post Translational Modifications | Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways. |
| Function | Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis. |
| Protein Name | B-Cell Linker ProteinB-Cell Adapter Containing A Sh2 Domain ProteinB-Cell Adapter Containing A Src Homology 2 Domain ProteinCytoplasmic Adapter ProteinSrc Homology 2 Domain-Containing Leukocyte Protein Of 65 KdaSlp-65 |
| Database Links | Reactome: R-HSA-912631Reactome: R-HSA-9679191Reactome: R-HSA-983695 |
| Cellular Localisation | CytoplasmCell MembraneBcr Activation Results In The Translocation To Membrane Fraction |
| Alternative Antibody Names | Anti-B-Cell Linker Protein antibodyAnti-B-Cell Adapter Containing A Sh2 Domain Protein antibodyAnti-B-Cell Adapter Containing A Src Homology 2 Domain Protein antibodyAnti-Cytoplasmic Adapter Protein antibodyAnti-Src Homology 2 Domain-Containing Leukocyte Protein Of 65 Kda antibodyAnti-Slp-65 antibodyAnti-BLNK antibodyAnti-BASH antibodyAnti-SLP65 antibody |
Information sourced from Uniprot.org