• Western blot analysis of extracts of various cell lines, using Integrin alpha V (ITGAV/CD51) (ITGAV/CD51) antibody (STJ11101608) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using Integrin alpha V (ITGAV/CD51) rabbit monoclonal antibody (STJ11101608) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Flow cytometry:1X10^6 Daudi cells (negative control, left) and HUVEC cells (right) were intracellularly-stained with Integrin alpha V (ITGAV/CD51) rabbit monoclonal antibody (STJ11101608, 2. 5 Mu g/mL, orange line) or rabbit IgG isotype control (2. 5 Mu g/mL, blue line) , followed by FITC conjugated goat anti-rabbit polyclonal antibody (1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
  • Flow cytometry:1X10^6 Daudi cells cells (negative control, left) and BEWO cells (right) were intracellularly-stained with Integrin alpha V (ITGAV/CD51) rabbit monoclonal antibody (STJ11101608, 2. 5 Mu g/mL, orange line) or rabbit IgG isotype control (2. 5 Mu g/mL, blue line) , followed by FITC conjugated goat anti-rabbit polyclonal antibody (1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
  • Flow cytometry:1X10^6 Daudi cells (negative control, left) and U-251MG cells (right) were intracellularly-stained with Integrin alpha V (ITGAV/CD51) rabbit monoclonal antibody (STJ11101608, 2. 5 Mu g/mL, orange line) or rabbit IgG isotype control (2. 5 Mu g/mL, blue line) , followed by FITC conjugated goat anti-rabbit polyclonal antibody (1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).

Anti-ITGAV antibody (949-1048) [S8MR] (STJ11101608)

SKU:
STJ11101608

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Integrin alpha V (949-1048) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
FC (Intra) 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGAV
Gene ID: 3685
Uniprot ID: ITAV_HUMAN
Immunogen Region: 949-1048
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 949-1048 of human Integrin alpha V (ITGAV/CD51) (NP_002201.2).
Immunogen Sequence: SLKSSASFNVIEFPYKNLPI EDITNSTLVTTNVTWGIQPA PMPVPVWVIILAVLAGLLLL AVLVFVMYRMGFFKRVRPPQ EEQEREQLQPHENGEGNSET
Function The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling. ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling. ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGAV:ITGB3 and ITGAV:ITGB6 act as receptors for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1. Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV:ITGB6 or ITGAV:ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation. ITGAV:ITGB3 acts as a receptor for CD40LG. (Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for Adenovirus type C. (Microbial infection) Integrin ITGAV:ITGB5 and ITGAV:ITGB3 act as receptors for Coxsackievirus A9 and B1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Herpes virus 8/HHV-8. (Microbial infection) Integrin ITGAV:ITGB6 acts as a receptor for herpes simplex 1/HHV-1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Human parechovirus 1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for West nile virus. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Protein Name Integrin Alpha-V
Vitronectin Receptor
Vitronectin Receptor Subunit Alpha
Cd Antigen Cd51 Cleaved Into - Integrin Alpha-V Heavy Chain - Integrin Alpha-V Light Chain
Database Links Reactome: R-HSA-1236973
Reactome: R-HSA-1566948
Reactome: R-HSA-210990
Reactome: R-HSA-2129379
Reactome: R-HSA-216083
Reactome: R-HSA-2173789
Reactome: R-HSA-3000157
Reactome: R-HSA-3000170
Reactome: R-HSA-3000178
Reactome: R-HSA-4420097
Reactome: R-HSA-445144
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Junction
Focal Adhesion
Alternative Antibody Names Anti-Integrin Alpha-V antibody
Anti-Vitronectin Receptor antibody
Anti-Vitronectin Receptor Subunit Alpha antibody
Anti-Cd Antigen Cd51 Cleaved Into - Integrin Alpha-V Heavy Chain - Integrin Alpha-V Light Chain antibody
Anti-ITGAV antibody
Anti-MSK8 antibody
Anti-VNRA antibody
Anti-VTNR antibody

Information sourced from Uniprot.org

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