• Western blot analysis of extracts of various cell lines, using CD8A rabbit monoclonal antibody (STJ11101251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of paraffin-embedded Human intestinal T-cell lymphoma using CD8A rabbit monoclonal antibody (STJ11101251) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human T-cell malignant lymphoma using CD8A rabbit monoclonal antibody (STJ11101251) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using CD8A rabbit monoclonal antibody (STJ11101251) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Flow cytometry: U937 cells were stained with rabbit IgG isotype control (10 Mu g/mL, blue line) or CD8A rabbit monoclonal antibody (STJ11101251, 10 Mu g/mL orange line) , followed by FITC conjugated goat anti-rabbit polyclonal antibody (1:200 dilution) staining. Non-fluorescently stained U937 cells were used as blank control (red line).
  • Flow cytometry: Jurkat cells were stained with rabbit IgG isotype control (10 Mu g/mL, blue line) or CD8A rabbit monoclonal antibody (STJ11101251, 10 Mu g/mL orange line) , followed by FITC conjugated goat anti-rabbit polyclonal antibody (1:200 dilution) staining. Non-fluorescently stained Jurkat cells were used as blank control (red line).
  • Flow cytometry: TALL-104 cells were stained with rabbit IgG isotype control (10 Mu g/mL, blue line) or CD8A rabbit monoclonal antibody (STJ11101251, 10 Mu g/mL orange line) , followed by FITC conjugated goat anti-rabbit polyclonal antibody (1:200 dilution) staining. Non-fluorescently stained TALL-104 cells were used as blank control (red line).

Anti-CD8A antibody (136-235) [S1MR] (STJ11101251)

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STJ11101251

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD8A (136-235) is suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
FC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD8A
Gene ID: 925
Uniprot ID: CD8A_HUMAN
Immunogen Region: 136-235
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 136-235 of human CD8A (P01732).
Immunogen Sequence: KPTTTPAPRPPTPAPTIASQ PLSLRPEACRPAAGGAVHTR GLDFACDIYIWAPLAGTCGV LLLSLVITLYCNHRNRRRVC KCPRPVVKSGDKPSLSARYV
Tissue Specificity CD8 on thymus-derived T-cells usually consists of a disulfide-linked alpha/CD8A and a beta/CD8B chain. Less frequently, CD8 can be expressed as a CD8A homodimer. A subset of natural killer cells, memory T-cells, intraepithelial lymphocytes, monocytes and dendritic cells expresses CD8A homodimers. Expressed at the cell surface of plasmacytoid dendritic cells upon herpes simplex virus-1 stimulation.
Post Translational Modifications Palmitoylated, but association with CD8B seems to be more important for the enrichment of CD8A in lipid rafts. O-glycosylated. Phosphorylated in cytotoxic T-lymphocytes (CTLs) following activation.
Function Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
Protein Name T-Cell Surface Glycoprotein Cd8 Alpha Chain
T-Lymphocyte Differentiation Antigen T8/Leu-2
Cd Antigen Cd8a
Database Links Reactome: R-HSA-198933
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Cd8a Localizes To Lipid Rafts Only When Associated With Its Partner Cd8b
Isoform 2: Secreted
Alternative Antibody Names Anti-T-Cell Surface Glycoprotein Cd8 Alpha Chain antibody
Anti-T-Lymphocyte Differentiation Antigen T8/Leu-2 antibody
Anti-Cd Antigen Cd8a antibody
Anti-CD8A antibody
Anti-MAL antibody

Information sourced from Uniprot.org

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