Primary Antibodies | St John's Laboratory

Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.

Anti-Vimentin antibody [2D1] (STJA0003824)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/D.melanogaster/Feline/Finch/Fish/Goat/Guinea Pig/Hamster/Horse/Lizard/Non-Human Primates/Rabbit/Sheep/Vole/Xenopus/Zebrafish

£454.50

Choose Options

Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~24 kDa UCHL1 protein.

Anti-UCHL1 antibody (Full Length) (STJA0003821)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Pig/Rat/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Vole

£403.50

Choose Options

Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.

Anti-TH antibody (STJA0003816)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC

Reactivity :

All Mammalian/Chicken

£454.50

Choose Options

Immunostaining of mouse dentate gyrus showing beta III tubulin (red, 1:1000). The blue is staining nuclei. Photo courtesy of Robert Wine.

Anti-TUBB3 antibody [AA10] (STJA0003815)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Mouse/Rat/Bovine/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Human/Non-Human Primates/Rabbit/Sheep/Vole

£454.50

Choose Options

Western blot of rat cortical lysate showing specific immunolabeling of the ~48 kDa, ~65 kDa & ~75 kDa tau isoforms.

Anti-MAPT antibody (Full Length) (STJA0003795)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Pig/Rat

£454.50

Choose Options

Western blot of 10ug of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.

Anti-SYN1 antibody (STJA0003781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

£454.50

Choose Options

Anti-SYN1 antibody (STJA0003780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

£454.50

Choose Options

Immunoflourescence of HeLa cells labeled with anti-SAP49 (STJA0003775, red, 1:1000) and anti-vimentin (STJA0003823, green, 1:10,000). The blue is DAPI staining nuclear DNA.

Anti-SF3B4 antibody (Full Length) [3A1] (STJA0003775)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat

£454.50

Choose Options

Immunoflourescence of mixed fibroblasts and PC12 cells labeled with anti-peripherin (STJA0003748, green, 1:5000) and anti-vimentin (red). The blue is DAPI staining nuclear DNA. Peripherin is labeled in only the PC12 cells cytoplasmic filaments and vimentin is only labeled in the fibroblasts intermediate filaments where peripherin is not present.

Anti-PRPH antibody (STJA0003748)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Rat/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep

£403.50

Choose Options

Immunostaining of cultured newborn rat neurons and glia showing peripherin (STJA0003747, green, 1:200) and neurofilament L (red). The nuclear DNA is stained blue.

Anti-PRPH antibody [7C5] (STJA0003747)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Mouse/Rat

£454.50

Choose Options

Western blot of rat cortical lysate showing specific immunolabeling of the ~145 kDa NF-M protein.

Anti-NEFM antibody (C-Term) (STJA0003702)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Chicken/Human/Mouse/Rat

£403.50

Choose Options

Anti-NEFM antibody (C-Term) [3H11] (STJA0003701)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Chicken/Human/Mouse/Rat

£403.50

Choose Options

Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.

Anti-NEFL antibody (STJA0003700)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Chicken/Human/Mouse/Rat

£403.50

Choose Options

Anti-NEFL antibody [DA2] (STJA0003699)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Chicken/Human/Mouse/Rat

£403.50

Choose Options

Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 kDa nestin doublet.

Anti-NES antibody [4D11] (STJA0003696)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat

£454.50

Choose Options

Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~70 kDa MAP2C/D proteins and the ~280 kDa MAP2A/B proteins.

Anti-MAP2 antibody (Full Length) [2C4] (STJA0003677)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Rat

£454.50

Choose Options

Western blot of HeLa cell lysate showing specific immunolabeling of the ~100 kDa LAMP1 protein.

Anti-LAMP1 antibody (Full Length) [5H6] (STJA0003675)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human

£454.50

Choose Options

Western blot of HeLa cell lysate showing specific immunolabeling of the ~64 kDa and ~74 kDa lamin A/C proteins.

Anti-LMNA antibody (Full Length) [4C4] (STJA0003674)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human

£454.50

Choose Options

Western blot of rat hippocampal lysate showing the specific immunolabeling of ~38 kDa GAPDH protein.

Anti-GAPDHS antibody [1D4] (STJA0003666)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Rat

£454.50

Choose Options

Western blot of rat cortical lysate showing specific immunolabeling of the ~43 kDa GAP43 protein.

Anti-GAP43 antibody (C-Term) (STJA0003656)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Horse/Human/Mouse/Rat

£403.50

Choose Options

Western blot of rat brain lysate showing specific immunolabeling of the ~53 kDa Beta 3-subunit of the GABAA-R.

Anti-GABRB3 antibody (Cytoplasmic Loop) (STJA0003647)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Mouse/Rat

£454.50

Choose Options

Western blot of HeLa cell lysate showing specific immunolabeling of the ~34 kDa fibrillarin protein.

Anti-NOP1 antibody [38F3] (STJA0003634)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Chicken/D.melanogaster/Human/Mouse/Pig/Rat/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Vole

£403.50

Choose Options

Western blot of postnatal day 3 rat brain lysate showing specific immunolabeling of the ~35 kDa and ~45 kDa doublecortin protein.

Anti-DCX antibody (Full Length) [3 e1] (STJA0003619)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Rat

£454.50

Choose Options

Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (STJA0003600, 1:100, red) that has formed fibrils in the extracellular matrix. The blue is staining nuclear DNA.

Anti-Collagen 1, alpha 1 telopeptide antibody (STJA0003600)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat/Sheep/Bovine/Canine/Chicken/Feline/Finch/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Vole/Xenopus

£454.50

Choose Options

Western blot of rat cerebellar lysate showing specific immunolabeling of the ~ 28 kDa calbindin protein.

Anti-CALB1 antibody (Full Length) (STJA0003587)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Bovine/Human/Mouse/Rat

£454.50

Choose Options

Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (No. STJA0003582, red, 1:1000). Nuclei labeled with DAPI. Magnification 10x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.

Anti-Phospho-AQP2-Ser 269 antibody (STJA0003582)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC/IP

Reactivity :

Human/Mouse/Rat/Bovine/Canine

£454.50

Choose Options

Immunofluorescence of a section of rat cerebellum showing labeling of axons of granule cell and dendrites of Purkinje with alpha-internexin (STJA0003573, 1:5000, red) , labeling of the myelin sheaths around the axons with MBP (STJA0003695, 1:5000, green) , and additional nuclear staining with DAPI (blue).

Anti-Alpha Internexin/NF-66 antibody (STJA0003573)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Rat

£403.50

Choose Options

Immunofluorescence of a section of rat cerebellum selectively labeling the axons of granule cells with alpha-internexin (STJA0003572, 1:5000, green) and colabeled with anti-calretinin (red).

Anti-Alpha Internexin/NF-66 antibody [1D2] (STJA0003572)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat

£403.50

Choose Options

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~240 kDa alpha II spectrin protein.

Anti-SPTAN1 antibody [3D7] (STJA0003570)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Rat

£454.50

Choose Options

Immunofluorescence of a section of rat hippocampus selectively labeling the neuronal cilia with anti-adenylate cyclase III (STJA0003569, 1:10,000, red) , labeling nuclei with anti-MECP2 (STJA0003679, 1:1000, green) , and Hoechst staining of nuclear DNA.

Anti-ADCY3 antibody (STJA0003569)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat

£454.50

Choose Options

Western blot analysis of various lysates using (STJ11103782) at1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-BRD4-T204 antibody [S2MR] (STJ11103782)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

Choose Options

Western blot analysis of lysates from NIH/3T3 cells using Phospho-PKR/EIF2AK2-T446 Rabbit pAb (STJ11103768) at 1:2000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-PKR/EIF2AK2-T446 antibody (STJ11103768)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

Choose Options

Western blot analysis of lysates from HT-29 cells, using Phospho-RIPK1/RIP-S166 Rabbit pAb (STJ11103757) at 1:800 dilution. HT-29 cells were treated with Z-VAD (50uM) , human TNF-Alpha (50ng/ml) and SM-164 (200nM) at 37 °C for 5 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-RIPK1/RIP-S166 antibody (STJ11103757)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

Choose Options

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CD117/c-Kit-Y721 Rabbit pAb (STJ11103718) at 1:500 dilution. NIH/3T3 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 20 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-CD117/cKit-Y719 antibody (STJ11103718)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of lysates from His Protein, using Rabbit anti His-tag mAb (STJ11103712) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-His-Tag antibody [S2MR] (STJ11103712)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Species independent

£207.50 - £392.50

Choose Options

Immunofluorescence analysis of NIH-3T3 cells using KIF3A Rabbit monoclonal antibody (STJ11103614) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-KIF3A antibody (600-699) [S4MR] (STJ11103614)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Immunofluorescence analysis of NIH-3T3 cells using Syntaxin 4 Rabbit monoclonal antibody (STJ11103604) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Syntaxin 4 antibody (50-150) [S4MR] (STJ11103604)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Confocal imaging of HeLa cells using 67kDa Laminin Receptor Rabbit monoclonal antibody (STJ11103600, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-67kDa Laminin Receptor antibody (196-295) [S0MR] (STJ11103600)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Immunofluorescence analysis of mouse brain cells using CEND1 Rabbit monoclonal antibody (STJ11103591) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CEND1 antibody (70-149) [S1MR] (STJ11103591)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Confocal imaging of NIH/3T3 cells using PIK3R4/VPS15 Rabbit monoclonal antibody (STJ11103589, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PIK3R4/VPS15 antibody (1259-1358) [S9MR] (STJ11103589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human appendix using VAMP8 Rabbit monoclonal antibody (STJ11103576) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-VAMP8 antibody (1-100) [S6MR] (STJ11103576)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human liver using L-Plastin/LCP1 Rabbit monoclonal antibody (STJ11103572) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-L-Plastin/LCP1 antibody (528-627) [S2MR] (STJ11103572)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£129.50 - £574.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using BNIP1 Rabbit monoclonal antibody (STJ11103564) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-BNIP1 antibody (50-150) [S4MR] (STJ11103564)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Confocal imaging of HeLa cells using PFDN5 Rabbit monoclonal antibody (STJ11103552, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PFDN5 antibody (50-154) [S2MR] (STJ11103552)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Flow cytometry: 1X10^6 Raji cells (negative control, left) and Jurkat cells (right) were intracellularly-stained with CD3G Rabbit monoclonal antibody (STJ11103551, 2. 5 Mu g/mL orange line) or ABflo® 488 Rabbit IgG isotype control ( 2. 5 Mu g/mL, blue line). Non-fluorescently stained cells were used as blank control (red line).

Anti-CD3G antibody (83-182) [S1MR] (STJ11103551)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Western blot analysis of various lysates, using AHR antibody (STJ11103544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AhR antibody (619-848) [S4MR] (STJ11103544)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

Choose Options

Immunoprecipitation analysis of 300 Mu g extracts of A-549 cells using 3 Mu g Kaiso/ZBTB33 antibody (STJ11103534). Western blot was performed from the immunoprecipitate using Kaiso/ZBTB33 antibody (STJ11103534) at a dilution of 1:1000.

Anti-Kaiso/ZBTB33 antibody (573-672) [S4MR] (STJ11103534)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Rat

£129.50 - £574.50

Choose Options

Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g HDAC6 antibody (STJ11103524). Western blot was performed from the immunoprecipitate using HDAC6 antibody (STJ11103524) at a dilution of 1:1000.

Anti-HDAC6 antibody (1-100) [S4MR] (STJ11103524)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human

£129.50 - £574.50

Choose Options