Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of JK cells using Phospho-GRK 2 (S685) Polyclonal Antibody diluted at 1：500

Anti-Phospho-GRK2-Ser685 antibody (640-689 aa) (STJ90616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of CACO2 lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-GRK2-Ser685 antibody (STJ99602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of 1 Jurkat cell, 2, LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-GRK2-Tyr13 antibody (STJA0010003)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-Phospho-GRK2-Tyr86 antibody (STJ193211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot WB GSK-3 phosphorylation in rabbit spleen fibroblasts using antibody STJA0041234

Anti-Phospho-GSK-3 Alpha/Beta-Tyr-216 antibody [M132] (STJA0041234)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Phospho-GSK3 antibody western blot 293 cells STJA0035670

Anti-Phospho-GSK3 alpha and beta-Tyr216/Tyr279 antibody [R47-6P-9] (STJA0035670)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various lysates using Phospho-GSK3 Beta-S9 Mouse mAb (STJ11107699) at 1:1000 dilution. HeLa NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-GSK3 Beta-S9 antibody [SMM5536] (STJ11107699)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Phospho-GSK3 beta Ser9 antibody western blot K562 C6 3T3 HeLa STJA0033924

Anti-Phospho-GSK3 beta-Ser9 antibody [R06-6H5] (STJA0033924)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of lysates from 293 and HeLa cells, using Phospho-GSK3 Alpha-S21 Rabbit pAb (STJ29348) at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin (0. 01U/mL) for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-GSK3A-S21 antibody (STJ29348)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of various cells using Phospho-GSK3 Alpha (S21) Polyclonal Antibody

Anti-Phospho-GSK3A-Ser21 antibody (10-59 aa) (STJ90282)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-GSK3A/B-Tyr-216 antibody [M132] (STJA0005434)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using GSK3β mouse monoclonal antibody diluted at 1:200.

Anti-Phospho-GSK3B antibody [6H6] (STJ196700)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of lysates from C6 cells using Phospho-GSK3 Beta-S9 Rabbit pAb (STJ22160) at 1:500 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-GSK3B-S9 antibody (STJ22160)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-GSK3 Beta-S9 Rabbit mAb (STJ11102623) at 1:1000 dilution incubated overnight at 4 °C. HeLa and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-GSK3B-S9 antibody [S3MR] (STJ11102623)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunofluorescence analysis of rat-spleen tissue. 1, GSK3 Beta (phospho Ser9) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-GSK3B-Ser9 antibody (1-50 aa) (STJ90284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat/D.melanogaster

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-GSK3 Beta (S9) Polyclonal Antibody diluted at 1：500

Anti-Phospho-GSK3B-Ser9 antibody (STJ90445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-GSK3B-Thr390 antibody (STJ196319)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-GSK3 Beta-Y216 + GSK3 Alpha-Y279 Rabbit pAb (STJ116370) at 1:1000 dilution. NIH/3T3 cells were treated by Lambda PP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-GSK3B-Y216/Y279 antibody (STJ116370)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from 293T cells, using Phospho-GSK3 Beta-Y216 + GSK3 Alpha-Y279 Rabbit pAb (STJ110852) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes or treated by 10% FBS for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-GSK3B-Y279 antibody (STJ110852)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from 1) 4T1, 2) 293 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5B7) (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-H2AX-Ser139 antibody (94-143 aa) (STJ90288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Hamster

£49.00 - £261.00

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Western blot analysis of extracts from Hela cells, 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-H2AX-Ser139 antibody (STJA0009882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.

Anti-Phospho-H2AX-Ser139 antibody [2A9-B5] (STJ99251)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse

£149.00 - £360.00

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Anti-Phospho-H2AX-Thr120 antibody (STJ197460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-H2AX-Tyr142 antibody (STJ197459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-H2BC3-Ser-36 antibody (STJA0005438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-H2BC9-Ser14 antibody (STJ194711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells, using Histone H3.3 (Phospho-Ser31) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-H3-3A/H3-3B-Ser31 antibody (16-65 aa) (STJ91330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-H4C1-Tyr-72 antibody (STJA0005439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Anti-Phospho-HDAC1-Ser406 antibody [R06-1W-1] (STJA0033026)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£224.50 - £319.50

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Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using HDAC1 (Phospho-Ser421) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-HDAC1-Ser421 antibody (387-436 aa) (STJ90497)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HepG2 cells, using Phospho-HDAC2-S394 Rabbit pAb (STJ11105771) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-HDAC2-S394 antibody (STJ11105771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various cells using Phospho-HDAC2 (S394) Polyclonal Antibody

Anti-Phospho-HDAC2-Ser394 antibody (360-409 aa) (STJ90442)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot HDAC2 phospho-Ser394 immunolabeling in mouse heart lysate STJA0003670

Anti-Phospho-HDAC2-Ser394 antibody (STJA0003670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat/Bovine/Chicken/Guinea Pig/Human/Non-Human Primates/Sheep

£488.50

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Immunofluorescence analysis of A549 cells, using HDAC3 (Phospho-Ser424) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-HDAC3-Ser424 antibody (379-428 aa) (STJ91081)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from 293 cells using Phospho-HDAC4-S632 Rabbit pAb (STJ22165). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-HDAC4-S632 antibody (STJ22165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of extracts of Jurkat cells, using Phospho-HDAC4-S632 antibody (STJ11105833) at1:2000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-HDAC4-S632 antibody [S5833RM] (STJ11105833)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of various cells using Phospho-HDAC4 (S632) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-HDAC4-Ser632 antibody (598-647 aa) (STJ90285)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-HDAC5-S498 Rabbit pAb (STJ22167) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-HDAC5-S498 antibody (STJ22167)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various cells using Phospho-HDAC5 (S498) Polyclonal Antibody diluted at 1：500

Anti-Phospho-HDAC5-Ser498 antibody (464-513 aa) (STJ90286)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Phospho-HDAC5-Ser498 antibody [PT1439R] (STJA0040328)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunofluorescence analysis of Hela cell. 1, HDAC6 (phospho Ser22) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Galectin-3 monoclonal antibody (6G2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-HDAC6-Ser22 antibody (7-56 aa) (STJ91082)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from HeLa cells, using HDAC7A (Phospho-Ser155) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-HDAC7-Ser155 antibody (121-170 aa) (STJ90655)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-HDAC7-Ser486 antibody (STJA0010043)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry of paraffin-embedded human lung carcinoma using Phospho-HDAC8-S39 antibody (STJ22168).

Anti-Phospho-HDAC8-S39 antibody (STJ22168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of various cells using Phospho-HDAC8 (S39) Polyclonal Antibody diluted at 1：500

Anti-Phospho-HDAC8-Ser39 antibody (5-54 aa) (STJ90287)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Hrs (Y216) Polyclonal Antibody

Anti-Phospho-HGS-Tyr216 antibody (STJ90292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using HRS (Phospho-Tyr334) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-HGS-Tyr334 antibody (301-350 aa) (STJ91095)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H2AX-S139 Rabbit pAb (STJ27513) at 1:400 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-Phospho-Histone H2A-S139 antibody (STJ27513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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