• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-HDAC5-S498 antibody (STJ22167) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Western blot analysis of extracts of NIH/3T3 cells, using Phospho-HDAC5-S498 antibody (STJ22167) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-HDAC5-S498 antibody (STJ22167).

Anti-Phospho-HDAC5-S498 antibody (STJ22167)

SKU:
STJ22167

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-HDAC5-S498 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDAC5
Gene ID: 10014
Uniprot ID: HDAC5_HUMAN
Immunogen: A synthetic phosphorylated peptide around S498 of human HDAC5 (NP_005465.2).
Immunogen Sequence: TQSSP
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylated by AMPK, CaMK1, SIK1 and PRKD1 at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm and inhibition. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylated by GRK5, leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Ubiquitinated. Polyubiquitination however does not lead to its degradation.
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response.
Protein Name Histone Deacetylase 5
Hd5
Antigen Ny-Co-9
Database Links Reactome: R-HSA-2122947
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-350054
Reactome: R-HSA-8943724
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between The Nucleus And The Cytoplasm
In Muscle Cells
It Shuttles Into The Cytoplasm During Myocyte Differentiation
The Export To Cytoplasm Depends On The Interaction With A 14-3-3 Chaperone Protein And Is Due To Its Phosphorylation At Ser-259 And Ser-498 By Ampk
Camk1 And Sik1
Alternative Antibody Names Anti-Histone Deacetylase 5 antibody
Anti-Hd5 antibody
Anti-Antigen Ny-Co-9 antibody
Anti-HDAC5 antibody
Anti-KIAA0600 antibody

Information sourced from Uniprot.org

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