• Immunohistochemistry analysis of paraffin-embedded human brain, using HRS (Phospho-Tyr334) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using HRS (Phospho-Tyr334) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HeLa cells treated with Forskolin 40nM 15', using HRS (Phospho-Tyr334) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-HGS-Tyr334 antibody (301-350 aa) (STJ91095)

SKU:
STJ91095

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Hepatocyte growth factor-regulated tyrosine kinase substrate-Tyr334 (301-350 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HGS
Gene ID: 9146
Uniprot ID: HGS_HUMAN
Immunogen Region: 301-350 aa
Specificity: Phospho-Hrs (Y334) Polyclonal Antibody detects endogenous levels of Hrs protein only when phosphorylated at Y334.
Immunogen: The antiserum was produced against synthesized peptide derived from the human HRS around the phosphorylation site of Tyr334 at the amino acid range 301-350
Post Translational Modifications Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected. Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF. Ubiquitinated. Ubiquitinated by ITCH.
Function Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
Protein Name Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate
Hrs
Protein Pp110
Database Links Reactome: R-HSA-182971
Reactome: R-HSA-432720
Reactome: R-HSA-5689880
Reactome: R-HSA-6807004
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8875360
Reactome: R-HSA-9013420
Reactome: R-HSA-917729
Reactome: R-HSA-9635644
Reactome: R-HSA-9636383
Reactome: R-HSA-9706019
Cellular Localisation Cytoplasm
Early Endosome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Endosome
Multivesicular Body Membrane
Colocalizes With Ubqln1 In Ubiquitin-Rich Cytoplasmic Aggregates That Are Not Endocytic Compartments
Alternative Antibody Names Anti-Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate antibody
Anti-Hrs antibody
Anti-Protein Pp110 antibody
Anti-HGS antibody
Anti-HRS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance