Anti-Phospho-H4C1-Tyr-72 antibody (STJA0005439)

SKU:
STJA0005439-100

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Host: Rabbit
Applications: WB
Reactivity: Chicken/Human/Mouse/Rat/Xenopus
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Histone H4-Tyr-72 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Purification: Antigen Affinity Purified
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H4C1.H4C2.H4C3.H4C4.H4C5.H4C6.H4C8.H4C9.H4C11.H4C12.H4C13.H4C14.H4C15.H4C16
Gene ID: 121504/554313/8294/8359/8360/8361/8362/8363/8364/8365/8366/8367/8368/8370
Uniprot ID: H4_HUMAN
Specificity: The antibody was cross adsorbed to unphosphorylated histone H4 (Tyr-72) peptide before affinity purification using phospho-histone H4 (Tyr-72) peptide. This antibody antibody detects a 10 kDa* protein on SDS-PAGE immunoblots of human PC3 and A431 cel
Immunogen: Phospho-Histone H4 (Tyr-72) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Tyr-72 in human histone H4. This site is well conserved in rat and mouse histone H4, but the site is not found in other histone family members
Post Translational Modifications Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by KMT5A/SET8. Dimethylation and trimethylation is performed by KMT5B and KMT5C and induces gene silencing. Monomethylated at Lys-13 (H4K12me1) by N6AMT1.H4K12me1 modification is present at the promoters of numerous genes encoding cell cycle regulators. Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Ufmylated.monofmylated by UFL1 at Lys-32 (H4K31Ufm1) in response to DNA damage. Sumoylated, which is associated with transcriptional repression. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Butyrylation of histones marks active promoters and competes with histone acetylation. Glutarylation at Lys-92 (H4K91glu) destabilizes nucleosomes by promoting dissociation of the H2A-H2B dimers from nucleosomes. Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription.
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Protein Name Histone H4
Database Links Reactome: R-HSA-110328
Reactome: R-HSA-110329
Reactome: R-HSA-110330
Reactome: R-HSA-110331
Reactome: R-HSA-1221632
Reactome: R-HSA-171306
Reactome: R-HSA-1912408
Reactome: R-HSA-201722
Reactome: R-HSA-212300
Reactome: R-HSA-2299718
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559586
Reactome: R-HSA-3214815
Reactome: R-HSA-3214841
Reactome: R-HSA-3214842
Reactome: R-HSA-3214847
Reactome: R-HSA-3214858
Reactome: R-HSA-427359
Reactome: R-HSA-427389
Reactome: R-HSA-427413
Reactome: R-HSA-4551638
Reactome: R-HSA-5250924
Reactome: R-HSA-5334118
Reactome: R-HSA-5578749
Reactome: R-HSA-5617472
Reactome: R-HSA-5625886
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-606279
Reactome: R-HSA-68616
Reactome: R-HSA-69473
Reactome: R-HSA-73728
Reactome: R-HSA-73772
Reactome: R-HSA-8936459
Reactome: R-HSA-8939236
Reactome: R-HSA-9018519
Reactome: R-HSA-912446
Reactome: R-HSA-9609690
Reactome: R-HSA-9610379
Reactome: R-HSA-9616222
Reactome: R-HSA-9670095
Reactome: R-HSA-9710421
Reactome: R-HSA-977225
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Histone H4 antibody
Anti-H4C1 antibody
Anti-H4 antibody
Anti-A antibody
Anti-H4FA antibody
Anti-HIST1H4A.H4C2 antibody
Anti-H4 antibody
Anti-I antibody
Anti-H4FI antibody
Anti-HIST1H4B.H4C3 antibody
Anti-H4 antibody
Anti-G antibody
Anti-H4FG antibody
Anti-HIST1H4C.H4C4 antibody
Anti-H4 antibody
Anti-B antibody
Anti-H4FB antibody
Anti-HIST1H4D.H4C5 antibody
Anti-H4 antibody
Anti-J antibody
Anti-H4FJ antibody
Anti-HIST1H4E.H4C6 antibody
Anti-H4 antibody
Anti-C antibody
Anti-H4FC antibody
Anti-HIST1H4F.H4C8 antibody
Anti-H4 antibody
Anti-H antibody
Anti-H4FH antibody
Anti-HIST1H4H.H4C9 antibody
Anti-H4 antibody
Anti-M antibody
Anti-H4FM antibody
Anti-HIST1H4I.H4C11 antibody
Anti-H4 antibody
Anti-E antibody
Anti-H4FE antibody
Anti-HIST1H4J.H4C12 antibody
Anti-H4 antibody
Anti-D antibody
Anti-H4FD antibody
Anti-HIST1H4K.H4C13 antibody
Anti-H4 antibody
Anti-K antibody
Anti-H4FK antibody
Anti-HIST1H4L.H4C14 antibody
Anti-H4 antibody
Anti-N antibody
Anti-H4F2 antibody
Anti-H4FN antibody
Anti-HIST2H4 antibody
Anti-HIST2H4A.H4C15 antibody
Anti-H4 antibody
Anti-O antibody
Anti-H4FO antibody
Anti-HIST2H4B.H4C16 antibody
Anti-H4-16 antibody
Anti-HIST4H4 antibody

Information sourced from Uniprot.org

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