• Western blot analysis of 3T3 cells using Phospho-HDAC2 (S394) Polyclonal Antibody
  • Western blot analysis of lysates from LOVO cells, using HDAC2 (Phospho-Ser394) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-HDAC2 (S394) Polyclonal Antibody

Anti-Phospho-HDAC2-Ser394 antibody (360-409 aa) (STJ90442)

SKU:
STJ90442

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Histone deacetylase 2-Ser394 (360-409 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDAC2
Gene ID: 3066
Uniprot ID: HDAC2_HUMAN
Immunogen Region: 360-409 aa
Specificity: Phospho-HDAC2 (S394) Polyclonal Antibody detects endogenous levels of HDAC2 protein only when phosphorylated at S394.
Immunogen: The antiserum was produced against synthesized peptide derived from the human HDAC2 around the phosphorylation site of Ser394 at the amino acid range 360-409
Post Translational Modifications S-nitrosylated by GAPDH. In neurons, S-nitrosylation at Cys-262 and Cys-274 does not affect enzyme activity, but induces HDAC2 release from chromatin. This in turn increases acetylation of histones surrounding neurotrophin-dependent gene promoters and promotes their transcription. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
Function Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Also deacetylates non-histone targets: deacetylates TSHZ3, thereby regulating its transcriptional repressor activity. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A. In addition to protein deacetylase activity, also acts as protein-lysine deacylase by recognizing other acyl groups: catalyzes removal of (2E)-butenoyl (crotonyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation and de-2-hydroxyisobutyrylation, respectively.
Protein Name Histone Deacetylase 2
Hd2
Protein Deacylase Hdac2
Database Links Reactome: R-HSA-193670
Reactome: R-HSA-2122947
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-3214815
Reactome: R-HSA-350054
Reactome: R-HSA-427389
Reactome: R-HSA-427413
Reactome: R-HSA-4551638
Reactome: R-HSA-6804758
Reactome: R-HSA-73762
Reactome: R-HSA-8943724
Reactome: R-HSA-9022692
Reactome: R-HSA-9022699
Reactome: R-HSA-9615017
Reactome: R-HSA-9619665
Reactome: R-HSA-9679191
Reactome: R-HSA-9701898
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Histone Deacetylase 2 antibody
Anti-Hd2 antibody
Anti-Protein Deacylase Hdac2 antibody
Anti-HDAC2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance