Western blot analysis of extracts from untreated or Hydroxyurea-treated Hela and A549 cells, using Histone H2A.X (Phospho-Ser139) mouse mAb (1:1000 diluted) (upper) or Beta-Actin Mouse mAb (200068-8F10) (lower).Predicted band size:15KDa.Observed band size:15KDa.

Anti-Phospho-H2AX-Ser139 antibody [2A9-B5] (STJ99251)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
STJ99251
🚚 Free UK Delivery on orders over £150
Processing The item has been added
Enquire For Bulk Order
✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short DescriptionMouse monoclonal anti-Phospho-Histone H2AX-Ser139 for use in WB, IHC and IF in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/IF
HostMouse
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone ID2A9-B5
IsotypeIgG2a
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:2000
IHC-P 1:100-500
ICC 1:400
IF 1:50-200
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolH2AX
Gene ID3014
Uniprot IDH2AX_HUMAN
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
SpecificityThis antibody detects endogenous levels of H2A.X only when phosphorylated at serine 139.

Additional Info

Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Protein Name Histone H2ax
H2a/X
Histone H2a.x
Database Links Reactome: R-HSA-110328
Reactome: R-HSA-110329
Reactome: R-HSA-110330
Reactome: R-HSA-110331
Reactome: R-HSA-1221632
Reactome: R-HSA-171306
Reactome: R-HSA-1912408
Reactome: R-HSA-201722
Reactome: R-HSA-212300
Reactome: R-HSA-2299718
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559586
Reactome: R-HSA-3214858
Reactome: R-HSA-427359
Reactome: R-HSA-427389
Reactome: R-HSA-427413
Reactome: R-HSA-5250924
Reactome: R-HSA-5334118
Reactome: R-HSA-5578749
Reactome: R-HSA-5617472
Reactome: R-HSA-5625886
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-606279
Reactome: R-HSA-68616
Reactome: R-HSA-69473
Reactome: R-HSA-73728
Reactome: R-HSA-73772
Reactome: R-HSA-8936459
Reactome: R-HSA-8939236
Reactome: R-HSA-9018519
Reactome: R-HSA-912446
Reactome: R-HSA-9616222
Reactome: R-HSA-9670095
Reactome: R-HSA-9710421
Reactome: R-HSA-977225
Reactome: R-HSA-9821002
Reactome: R-HSA-9821993
Reactome: R-HSA-9841922
Reactome: R-HSA-9843940
Reactome: R-HSA-9843970
Reactome: R-HSA-9845323
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Histone H2ax antibody
Anti-H2a/X antibody
Anti-Histone H2a.x antibody
Anti-H2AX antibody
Anti-H2AFX antibody

Information sourced from Uniprot.org

Citations

Product Review

!