• Western blot analysis of HELA SH-SY5Y 3T3 lysis using Phospho-HDAC4 (S632) antibody. Antibody was diluted at 1:1000
  • Western blot analysis of lysates from 293 cells treated with etoposide 25uM 1hour and Jurkat cells treated with etoposide 25uM 24hours, using HDAC4 (Phospho-Ser632) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-HDAC4 (S632) Polyclonal Antibody diluted at 1:1000

Anti-Phospho-HDAC4-Ser632 antibody (598-647 aa) (STJ90285)

SKU:
STJ90285

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Histone deacetylase 4-Ser632 (598-647 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDAC4
Gene ID: 9759
Uniprot ID: HDAC4_HUMAN
Immunogen Region: 598-647 aa
Specificity: Phospho-HDAC4 (S632) Polyclonal Antibody detects endogenous levels of HDAC4 protein only when phosphorylated at S632.
Immunogen: The antiserum was produced against synthesized peptide derived from the human HDAC4 around the phosphorylation site of Ser632 at the amino acid range 598-647
Post Translational Modifications Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues by CaMK2D is required for the interaction with 14-3-3. Phosphorylation at Ser-350, within the PxLPxI/L motif, impairs the binding of ANKRA2 but generates a high-affinity docking site for 14-3-3. Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1.
Protein Name Histone Deacetylase 4
Hd4
Database Links Reactome: R-HSA-2122947
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-350054
Reactome: R-HSA-4090294
Reactome: R-HSA-4551638
Reactome: R-HSA-8941284
Reactome: R-HSA-8951936
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between The Nucleus And The Cytoplasm
Upon Muscle Cells Differentiation
It Accumulates In The Nuclei Of Myotubes
Suggesting A Positive Role Of Nuclear Hdac4 In Muscle Differentiation
The Export To Cytoplasm Depends On The Interaction With A 14-3-3 Chaperone Protein And Is Due To Its Phosphorylation At Ser-246
Ser-467 And Ser-632 By Camk4 And Sik1
The Nuclear Localization Probably Depends On Sumoylation
Interaction With Sik3 Leads To Hdac4 Retention In The Cytoplasm
Alternative Antibody Names Anti-Histone Deacetylase 4 antibody
Anti-Hd4 antibody
Anti-HDAC4 antibody
Anti-KIAA0288 antibody

Information sourced from Uniprot.org

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