Reproduction | Research Area | St John's Laboratory

Anti-Phospho-AKT1-S473 antibody [7W3-W4-S4] (STJA0033682)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ChIP/IP

Reactivity :

Human

$309.81 - $440.91

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Western blot analysis of various lysates using (STJ22028) at 1:1000 dilution. 293T cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours. 293T cells were treated by UV at room temperature for 15-30 minutes. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-ATM-S1981 antibody (STJ22028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Confocal imaging of paraffin-embedded Human colon tissue (untreated) and Human colon tissue (treated with Lambda pp) using Phospho-ATM-S1981 Rabbit mAb (STJ11102610, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-Phospho-ATM-S1981 antibody [S0MR] (STJ11102610)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$163.53 - $719.67

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Anti-Phospho-ATM-Ser-1981 antibody [M366] (STJA0005274)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human/Mouse/Rat

$504.39

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Anti-Phospho-ATM-Ser-1981 antibody [M366] (STJA0041054)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IP/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-ATM-Ser-794 antibody (STJA0005273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/FC

Reactivity :

Human/Mouse/Rat

$504.39

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Anti-Phospho-ATM-Ser-794 antibody (STJA0041068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC/FC

Reactivity :

Human/Mouse/Rat

$748.65

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Western Blot analysis of 1 K562 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ATM-Ser179 antibody (STJA0010053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of K562 using p-Atm (S1981) antibody. Antibody was diluted at 1:500

Anti-Phospho-ATM-Ser1981 antibody (STJ97691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-ATM-Ser1981 antibody [PT0902R] (STJA0039795)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Anti-Phospho-ATM-Ser1981 antibody [R01-5I8] (STJA0035784)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human

$184.23 - $388.47

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Western Blot analysis of mouse heart, Hela, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ATM-Ser367 antibody (STJA0010035)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from 293T cells, using Phospho-ATR-S428 Rabbit pAb (STJ114848) at 1:400 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-ATR-S428 antibody (STJ114848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of various lysates, using Phospho-ATR-S428 antibody (STJ11105842) at1:2000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-ATR-S428 antibody [S5842RM] (STJ11105842)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Anti-Phospho-ATR-S428-S428 antibody [SRM15157] (STJ11109887)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using ATR (Phospho-Ser428) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATR-Ser428 antibody (394-443 aa) (STJ91234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-Phospho-ATR-Ser428 antibody [PT1243R] (STJA0040115)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Western blot analysis of lysates from HeLa cells, using Phospho-ATR-T1989 Rabbit pAb (STJ11103765) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-ATR-T1989 antibody (STJ11103765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Anti-Phospho-ATR-Thr1989 antibody (STJ196248)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BLM-Thr99 antibody (65-114 aa) (STJ91066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from HT-29 cells, using Phospho-BRCA1-S1423 Rabbit pAb (STJ22043) at 1:2000 dilution. HT-29 cells were treated by Anisomycin (5 Mu g/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-BRCA1-S1423 antibody (STJ22043)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Phospho-BRCA1-S988 Rabbit pAb (STJ22045) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-Phospho-BRCA1-S988 antibody (STJ22045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human

$174.57 - $853.53

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BRCA1 (Phospho-Ser1423) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BRCA1-Ser1423 antibody (1391-1440 aa) (STJ90198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

$67.62 - $360.18

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Western blot analysis of lysates from 293 cells treated with epo 20U/ml 15', using BRCA1 (Phospho-Ser1457) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-BRCA1-Ser1457 antibody (1423-1472 aa) (STJ90622)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western Blot analysis of mouse brain rat brain, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-BRCA1-Ser1497 antibody (STJA0010038)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BRCA1 (Phospho-Ser1524) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BRCA1-Ser1524 antibody (1491-1540 aa) (STJ90199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-Phospho-BRCA1-Ser988 antibody (STJA0009869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-Phospho-BRCA2-Ser3291 antibody (STJ90672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from C6 cells, using Phospho-CDK2-T160 Rabbit pAb (STJ22062) at 1:400 dilution. C6 cells were treated by Nocodazole (10 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CDK2-T160 antibody (STJ22062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from C6 cells using Phospho-CDK2-T160 Rabbit monoclonal antibody (STJ11105902) at 1:1000 dilution. C6 cells were treated by Nocodazole (10ng/ml). Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-CDK2-T160 antibody [S5902RM] (STJ11105902)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$198.03 - $853.53

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Anti-Phospho-CDK2-T160-T160 antibody [SRM15122] (STJ11109852)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-CDK2-Thr14 antibody [R06-4C1] (STJA0035056)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

$184.23 - $388.47

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Western blot analysis of various cells using Phospho-Cdk2/Cdc2 (T160) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CDK2-Thr160 antibody (STJ90752)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-CDK2-Tyr15 antibody [R05-2C4] (STJA0033906)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Hamster/Rat

$184.23 - $388.47

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK2-Y15 Rabbit mAb (STJ11102594) at 1:1000 dilution. NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37 °C for 20 hours or treated by Nocodazole (100 ng/mL) at 37 °C for 24 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-CDK2-Y15 antibody [S4MR] (STJ11102594)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$163.53 - $719.67

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK4-T172 Rabbit pAb (STJ23055). NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-CDK4-T172 antibody (STJ29359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Anti-Phospho-CDK4-Thr172 antibody (STJA0009886)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-CXCR4-S324/S325 antibody (C-Term) (STJA0005364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IHC

Reactivity :

Chicken/Human/Mouse/Rat

$504.39

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Anti-Phospho-CXCR4-S324/S325 antibody (C-Term) (STJA0041168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC/IHC

Reactivity :

Chicken/Human/Mouse/Rat

$748.65

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Immunofluorescence analysis of Hela cell. 1, Fusin (phospho Ser339) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-CXCR4-Ser339 antibody (303-352 aa) (STJ91077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Anti-Phospho-Gamma-H2AX-S139 antibody [9F3] (STJA0019679)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

$269.79 - $1,820.91

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Anti-Phospho-Gamma-H2AX-S139 antibody [A4] (STJA0019153)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$269.79 - $1,820.91

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Anti-Phospho-Gamma-H2AX-S139 antibody [A9] (STJA0019152)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$269.79 - $1,820.91

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Anti-Phospho-Gamma-H2AX-S139 antibody [C6] (STJA0019151)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$269.79 - $1,820.91

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Anti-Phospho-Gamma-H2AX-S139 antibody [G2] (STJA0019154)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC/IP/WB

Reactivity :

Human

$269.79 - $1,820.91

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Western blot analysis of lysates from 1) 4T1, 2) 293 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5B7) (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-H2AX-Ser139 antibody (94-143 aa) (STJ90288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Hamster

$67.62 - $360.18

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Western blot analysis of extracts from Hela cells, 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-H2AX-Ser139 antibody (STJA0009882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.

Anti-Phospho-H2AX-Ser139 antibody [2A9-B5] (STJ99251)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse

$205.62 - $496.80

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