Anti-Phospho-ATM-Ser-1981 antibody [M366] (STJA0005274)

SKU:
STJA0005274-100

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Host: Mouse
Applications: WB/IP/ICC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Phospho-ATM-Ser-1981 is suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: M366
Conjugation: Unconjugated
Isotype: IgG1
Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Purification: Protein A Purified
Dilution Range: WB 1:1000
IHC
ICC 1:200
IP 1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATM
Gene ID: 472
Uniprot ID: ATM_HUMAN
Specificity: This antibody detects a 370 kDa* protein corresponding to the molecular mass of ATM on SDS-PAGE immunoblots of calyculin A treated human A431 and Jurkat Cells, but is not observed in control cells.
Immunogen: Clone M366 was generated from a phospho-peptide that included amino acids surrounding Serine 1981 in human ATM. This sequence has high homology to the conserved site in rat and mouse ATM.
Tissue Specificity Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
Post Translational Modifications Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase. During the late stages of DNA damage response, dephosphorylated following deacetylation by SIRT7, leading to ATM deactivation. Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60. Deacetylated by SIRT7 during the late stages of DNA damage response, promoting ATM dephosphorylation and subsequent deactivation.
Function Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence ST-Q. Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FBXW7, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, UFL1, RAD9, UBQLN4 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks. Phosphorylates TTC5/STRAP at 'Ser-203' in the cytoplasm in response to DNA damage, which promotes TTC5/STRAP nuclear localization. Also involved in pexophagy by mediating phosphorylation of PEX5: translocated to peroxisomes in response to reactive oxygen species (ROS), and catalyzes phosphorylation of PEX5, promoting PEX5 ubiquitination and induction of pexophagy.
Protein Name Serine-Protein Kinase Atm
Ataxia Telangiectasia Mutated
A-T Mutated
Database Links Reactome: R-HSA-2559586
Reactome: R-HSA-3371453
Reactome: R-HSA-349425
Reactome: R-HSA-5685938
Reactome: R-HSA-5685942
Reactome: R-HSA-5693548
Reactome: R-HSA-5693554
Reactome: R-HSA-5693565
Reactome: R-HSA-5693568
Reactome: R-HSA-5693571
Reactome: R-HSA-5693579
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6796648
Reactome: R-HSA-6803204
Reactome: R-HSA-6803207
Reactome: R-HSA-6804756
Reactome: R-HSA-6804757
Reactome: R-HSA-6804760
Reactome: R-HSA-69473
Reactome: R-HSA-69541
Reactome: R-HSA-912446
Reactome: R-HSA-9664873
Reactome: R-HSA-9701192
Reactome: R-HSA-9704331
Reactome: R-HSA-9704646
Reactome: R-HSA-9709570
Reactome: R-HSA-9709603
Cellular Localisation Nucleus
Cytoplasmic Vesicle
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Peroxisome Matrix
Primarily Nuclear
Found Also In Endocytic Vesicles In Association With Beta-Adaptin
Translocated To Peroxisomes In Response To Reactive Oxygen Species (Ros) By Pex5
Alternative Antibody Names Anti-Serine-Protein Kinase Atm antibody
Anti-Ataxia Telangiectasia Mutated antibody
Anti-A-T Mutated antibody
Anti-ATM antibody

Information sourced from Uniprot.org

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