• Western blot analysis of 293T, using Phospho-ATR-S428 antibody (STJ114848) at 1:400 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of NIH/3T3, using Phospho-ATR-S428 antibody (STJ114848) at 1:400 dilution. NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human testis using Phospho-ATR-S428 rabbit polyclonal antibody (STJ114848) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using Phospho-ATR-S428 rabbit polyclonal antibody (STJ114848) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using Phospho-ATR-S428 rabbit polyclonal antibody (STJ114848) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U2OS cells using Phospho-ATR-S428 antibody (STJ114848) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-Phospho-ATR-S428 antibody (STJ114848)

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STJ114848

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-ATR-S428 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATR
Gene ID: 545
Uniprot ID: ATR_HUMAN
Immunogen: A synthetic phosphorylated peptide around S428 of human ATR (NP_001175.2).
Immunogen Sequence: NSDGISPKRR
Tissue Specificity Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
Post Translational Modifications Phosphorylated.autophosphorylates in vitro.
Function Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence ST-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex.
Protein Name Serine/Threonine-Protein Kinase Atr
Ataxia Telangiectasia And Rad3-Related Protein
Frap-Related Protein 1
Database Links Reactome: R-HSA-1221632
Reactome: R-HSA-176187
Reactome: R-HSA-3371453
Reactome: R-HSA-5685938
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6783310
Reactome: R-HSA-6796648
Reactome: R-HSA-6804756
Reactome: R-HSA-69473
Reactome: R-HSA-9709570
Cellular Localisation Nucleus
Chromosome
Depending On The Cell Type
It Can Also Be Found In Pml Nuclear Bodies
Recruited To Chromatin During S-Phase
Redistributes To Discrete Nuclear Foci Upon Dna Damage
Hypoxia Or Replication Fork Stalling
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Atr antibody
Anti-Ataxia Telangiectasia And Rad3-Related Protein antibody
Anti-Frap-Related Protein 1 antibody
Anti-ATR antibody
Anti-FRP1 antibody

Information sourced from Uniprot.org

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