• Immunohistochemistry analysis of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from HepG2 cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HeLa cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BLM-Thr99 antibody (65-114 aa) (STJ91066)

SKU:
STJ91066

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-RecQ-like DNA helicase BLM-Thr99 (65-114 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BLM
Gene ID: 641
Uniprot ID: BLM_HUMAN
Immunogen Region: 65-114 aa
Specificity: Phospho-BLM (T99) Polyclonal Antibody detects endogenous levels of BLM protein only when phosphorylated at T99.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Bloom Syndrome around the phosphorylation site of Thr99 at the amino acid range 65-114
Function ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. Participates in DNA replication and repair. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction. Recruited by the KHDC3L-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks. (Microbial infection) Eliminates nuclear HIV-1 cDNA, thereby suppressing immune sensing and proviral hyper-integration.
Protein Name Recq-Like Dna Helicase Blm
Bloom Syndrome Protein
Dna Helicase - Recq-Like Type 2
Recq2
Recq Protein-Like 3
Database Links Reactome: R-HSA-174414
Reactome: R-HSA-3108214
Reactome: R-HSA-5685938
Reactome: R-HSA-5685942
Reactome: R-HSA-5693554
Reactome: R-HSA-5693568
Reactome: R-HSA-5693579
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6804756
Reactome: R-HSA-69473
Reactome: R-HSA-912446
Reactome: R-HSA-9701192
Reactome: R-HSA-9704331
Reactome: R-HSA-9704646
Reactome: R-HSA-9709570
Reactome: R-HSA-9709603
Cellular Localisation Nucleus
Together With Spidr
Is Redistributed In Discrete Nuclear Dna Damage-Induced Foci Following Hydroxyurea (Hu) Or Camptothecin (Cpt) Treatment
Accumulated At Sites Of Dna Damage In A Rmi Complex- And Spidr-Dependent Manner
Alternative Antibody Names Anti-Recq-Like Dna Helicase Blm antibody
Anti-Bloom Syndrome Protein antibody
Anti-Dna Helicase - Recq-Like Type 2 antibody
Anti-Recq2 antibody
Anti-Recq Protein-Like 3 antibody
Anti-BLM antibody
Anti-RECQ2 antibody
Anti-RECQL3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance