| Host: |
Rabbit |
| Applications: |
IHC/IF/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase ATR-Ser428 (394-443 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ATR |
| Gene ID: |
545 |
| Uniprot ID: |
ATR_HUMAN |
| Immunogen Region: |
394-443 aa |
| Specificity: |
Phospho-ATR (S428) Polyclonal Antibody detects endogenous levels of ATR protein only when phosphorylated at S428. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human ATR around the phosphorylation site of Ser428 at the amino acid range 394-443 |
| Post Translational Modifications | Phosphorylated.autophosphorylates in vitro. |
| Function | Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence ST-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex. |
| Protein Name | Serine/Threonine-Protein Kinase AtrAtaxia Telangiectasia And Rad3-Related ProteinFrap-Related Protein 1 |
| Database Links | Reactome: R-HSA-1221632Reactome: R-HSA-176187Reactome: R-HSA-3371453Reactome: R-HSA-5685938Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6783310Reactome: R-HSA-6796648Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9709570 |
| Cellular Localisation | NucleusChromosomeDepending On The Cell TypeIt Can Also Be Found In Pml Nuclear BodiesRecruited To Chromatin During S-PhaseRedistributes To Discrete Nuclear Foci Upon Dna DamageHypoxia Or Replication Fork Stalling |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Atr antibodyAnti-Ataxia Telangiectasia And Rad3-Related Protein antibodyAnti-Frap-Related Protein 1 antibodyAnti-ATR antibodyAnti-FRP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
