• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-CDK4-T172 pAb (STJ29359) at 1:1000 dilution or CDK4 antibody (STJ23055).NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 60s.

Anti-Phospho-CDK4-T172 antibody (STJ29359)

SKU:
STJ29359

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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-Phospho-CDK4-T172 antibody is suitable for use in Western Blot.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Uniprot ID: CDK4_HUMAN
Gene ID: 1019
Gene Symbol: CDK4
Immunogen: A synthetic phosphorylated peptide around T172 of human CDK4 (NP_000066.1).
Post Translational Modifications Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
Function Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Protein Name Cyclin-Dependent Kinase 4
Cell Division Protein Kinase 4
Psk-J3
Database Links Reactome: R-HSA-187577
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559585
Reactome: R-HSA-3214858
Reactome: R-HSA-381340
Reactome: R-HSA-69231
Reactome: R-HSA-75815
Reactome: R-HSA-8849470
Reactome: R-HSA-8878166
Reactome: R-HSA-912446
Reactome: R-HSA-9616222
Reactome: R-HSA-9630791
Reactome: R-HSA-9630794
Reactome: R-HSA-9632697
Reactome: R-HSA-9632700
Reactome: R-HSA-9661069
Cellular Localisation Cytoplasm
Nucleus
Nucleus Membrane
Cytoplasmic When Non-Complexed
Forms A Cyclin D-Cdk4 Complex In The Cytoplasm As Cells Progress Through G(1) Phase
The Complex Accumulates On The Nuclear Membrane And Enters The Nucleus On Transition From G(1) To S Phase
Also Present In Nucleoli And Heterochromatin Lumps
Colocalizes With Rb1 After Release Into The Nucleus
Alternative Antibody Names Anti-Cyclin-Dependent Kinase 4 antibody
Anti-Cell Division Protein Kinase 4 antibody
Anti-Psk-J3 antibody
Anti-CDK4 antibody

Information sourced from Uniprot.org

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