Muscle Contraction | Research Area | St John's Laboratory

Western blot analysis of lysates from 293T cells, using Phospho-Na+/K+-ATPase-S16 Rabbit pAb (STJ11101103) at 1:1000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-ATP1A1-S16 antibody (STJ11101103)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using ATPase (Phospho-Ser16) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATP1A1-Ser16 antibody (5-54 aa) (STJ90804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of KB Hela lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-ATP1A1-Tyr260 antibody (230-290 aa) (STJ98679)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-ATP1A1-Tyr260 antibody (STJ99603)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-Phospho-CACNA1C-Ser1981 antibody (STJA0009931)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-CALD1-Ser759 antibody (725-774 aa) (STJ91244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various cells using Phospho-Caldesmon (S789) Polyclonal Antibody

Anti-Phospho-CALD1-Ser789 antibody (744-793 aa) (STJ91034)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from Mouse brain using Phospho-Camk2a-T286 Rabbit mAb (STJ11107739) at 1:5000 dilution incubated overnight at 4C. Mouse brain were treated by CIP (20uL/400ul) at 37C for 1 hour. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Enhanced Kit <br>Exposure time: 180s.

Anti-Phospho-Camk2a-T286 antibody (300-400 aa) [SRM5548] (STJ11107739)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€179.38 - €773.13

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Western blot analysis of lysates from Mouse brain, using Phospho-Camk2-T286 Rabbit pAb (STJ22047) at 1:4000 dilution. Mouse brain cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-Camk2a-T286 antibody (STJ22047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Desmin (Phospho-Ser60) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-DES-Ser60 antibody (26-75 aa) (STJ91182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of HepG2 cells, using GATA4 (Phospho-Ser105) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-GATA4-Ser105 antibody (71-120 aa) (STJ91340)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from 293 cells, using GATA4 (Phospho-Ser262) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-GATA4-Ser262 antibody (228-277 aa) (STJ90714)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-Phospho-HIPK1-Tyr352/361/359 antibody (STJA0009959)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma, using Inositol 1, 4, 5-trisphosphate R1 (Phospho-Ser1598/1588) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ITPR1-Ser1598 antibody (1566-1615 aa) (STJ91314)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-ITPR1-Ser1756 antibody (STJ196331)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of Rat-brain cells using Phospho-IP3R-I (S1764) Polyclonal Antibody

Anti-Phospho-ITPR1-Ser1764 antibody (1730-1779 aa) (STJ91258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-ITPR1-Tyr353 antibody (STJA0009850)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-KCND2-Ser616 antibody (STJ91165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of SH-SY5Y 293T 3T3 lysis using Phospho-DREAM (S63) antibody. Antibody was diluted at 1:500

Anti-Phospho-KCNIP3-Ser63 antibody (29-78 aa) (STJ91090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-KCNJ11-Thr224 antibody (190-239 aa) (STJ90722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-Phospho-MYL12A-Ser-1 antibody (STJA0005479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat/Bovine

€459.38

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Anti-Phospho-MYL12A-Ser-19 antibody (STJA0005480)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

€459.38

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Western blot analysis of lysates from C2C12 cells, using Phospho-MYL9-S19 Rabbit pAb (STJ11101010) at 1:400 dilution. C2C12 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-MYL9-S19 antibody (STJ11101010)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of C2C12, using Phospho-Myosin Light Chain 2-S19 Rabbit monoclonal antibody (STJ11105886) at 1:1000 dilution. C2C12 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-Phospho-MYL9-S19 antibody [S5886RM] (STJ11105886)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€148.13 - €651.88

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Immunohistochemistry analysis of paraffin-embedded human brain, using Myosin regulatory light chain 2 (Phospho-Ser18) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MYL9-Ser18 antibody (3-52 aa) (STJ91061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of HeLa, using Phospho-Myosin Light Chain 2-T18 Rabbit monoclonal antibody (STJ11105890) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-Phospho-MYL9-T18 antibody [S5890RM] (STJ11105890)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€148.13 - €651.88

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Western blot analysis of lysates from HeLa cells, using Phospho-MYL9-T18/S19 Rabbit pAb (STJ11101086) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-MYL9-T18/S19 antibody (STJ11101086)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of HeLa, using Phospho-Myosin Light Chain 2-T18/S19 Rabbit monoclonal antibody (STJ11105904) at 1:6000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-MYL9-T18/S19 antibody [S5904RM] (STJ11105904)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€148.13 - €651.88

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Anti-Phospho-MYL9-Thr18/Ser19 antibody [PT0752R] (STJA0039142)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€356.25 - €675.00

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-Phospho-MYLK-Tyr464 antibody (STJ91279)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Anti-Phospho-MYLK-Tyr471 antibody (STJA0009855)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemistry analysis of paraffin-embedded human brain, using n-NOS (Phospho-Ser852) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-NOS1-Ser852 antibody (818-867 aa) (STJ90355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€61.25 - €326.25

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Western blot analysis of lysates from LOVO cells treated with starved 24h, using PAK1 (Phospho-Ser199) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PAK1-Ser199 antibody (165-214 aa) (STJ90917)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€61.25 - €326.25

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Anti-Phospho-PAK1-Ser199/204 antibody (STJ196376)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-Phospho-PAK1-Ser20 antibody (STJ196377)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of 3T3 cells using Phospho-PAK Alpha (S204) Polyclonal Antibody

Anti-Phospho-PAK1-Ser204 antibody (170-219 aa) (STJ90954)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western Blot analysis of Raji cell, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PAK1-Ser21 antibody (STJA0010056)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of 293 cells using Phospho-PAK Alpha (T212) Polyclonal Antibody

Anti-Phospho-PAK1-Thr212 antibody (178-227 aa) (STJ90826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68 kDa PAK1 protein phosphorylated at Thr84 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase ( Lambda-Ptase, 800 units/1mg protein for 30 minutes).

Anti-Phospho-PAK1-Thr84 antibody (STJA0003738)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Guinea Pig/Non-Human Primates/Sheep/Zebrafish

€568.13

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-PAK2-S20 Rabbit pAb (STJ117901) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-PAK2-S20 antibody (STJ117901)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of C6, using Phospho-PAK2-S20 Rabbit monoclonal antibody (STJ11105893) at 1:2000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-PAK2-S20 antibody [S5893RM] (STJ11105893)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€148.13 - €651.88

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Western blot analysis of lysates from HepG2 cells treated with Adriamycin 0.5uM 24h/Jurkat cells treated with PMA 125ng/ml 30', using PAK2 (Phospho-Ser141) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PAK2-Ser141 antibody (107-156 aa) (STJ90590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from HeLa cells, using PAK2 (Phospho-Ser192) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PAK2-Ser192 antibody (158-207 aa) (STJ90589)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PAK2 (Phospho-Ser197) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PAK2-Ser197 antibody (163-212 aa) (STJ91214)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of 293 cells using Phospho-PAK Gamma (S20) Polyclonal Antibody

Anti-Phospho-PAK2-Ser20 antibody (5-54 aa) (STJ90860)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-Phospho-PGCA antibody (500-550) (STJ502627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-Phospho-PGCA antibody (500-550) {Biotin} (STJ502628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€730.63

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Anti-Phospho-PGCA antibody (500-550) {FITC} (STJ502629)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€730.63

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