• Immunofluorescence analysis of U2OS cells using MYL3 antibody (STJ28745). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using MYL3 antibody (STJ28745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-MYL3 antibody (1-195) (STJ28745)

SKU:
STJ28745

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MYL3 (1-195) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYL3
Gene ID: 4634
Uniprot ID: MYL3_HUMAN
Immunogen Region: 1-195
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human MYL3 (NP_000249.1).
Immunogen Sequence: MAPKKPEPKKDDAKAAPKAA PAPAPPPEPERPKEVEFDAS KIKIEFTPEQIEEFKEAFML FDRTPKCEMKITYGQCGDVL RALGQNPTQAEVLRVLGKPR QEELNTKMMDFETFLPMLQH ISKNKDTGTYEDFVEGLRVF DKEGNGTVMGAELRHVLATL GERLTEDEVEKLMAGQEDSN GCINYEAFVKHIMSS
Post Translational Modifications The N-terminus is blocked. N-terminus is methylated by METTL11A/NTM1.
Function Regulatory light chain of myosin. Does not bind calcium.
Protein Name Myosin Light Chain 3
Cardiac Myosin Light Chain 1
Cmlc1
Myosin Light Chain 1 - Slow-Twitch Muscle B/Ventricular Isoform
Mlc1sb
Ventricular Myosin Alkali Light Chain
Ventricular Myosin Light Chain 1
Vlcl
Ventricular/Slow Twitch Myosin Alkali Light Chain
Mlc-Lv/Sb
Database Links Reactome: R-HSA-390522
Alternative Antibody Names Anti-Myosin Light Chain 3 antibody
Anti-Cardiac Myosin Light Chain 1 antibody
Anti-Cmlc1 antibody
Anti-Myosin Light Chain 1 - Slow-Twitch Muscle B/Ventricular Isoform antibody
Anti-Mlc1sb antibody
Anti-Ventricular Myosin Alkali Light Chain antibody
Anti-Ventricular Myosin Light Chain 1 antibody
Anti-Vlcl antibody
Anti-Ventricular/Slow Twitch Myosin Alkali Light Chain antibody
Anti-Mlc-Lv/Sb antibody
Anti-MYL3 antibody

Information sourced from Uniprot.org

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