Anti-MYL2 antibody (67-166) [S6MR] (STJ11102366)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Myosin Light Chain 2 (67-166) antibody for use in WB, IF, ICC and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S6MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000IF/ICC:1:100-1:400ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MYL2 |
| Gene ID | 4633 |
| Uniprot ID | MLRV_HUMAN |
| Immunogen Region | 67-166 |
| Immunogen Sequence | DEMIKEAPGPINFTVFLTMF GEKLKGADPEETILNAFKVF DPEGKGVLKADYVREMLTTQ AERFSKEEVDQMFAAFPPDV TGNLDYKNLVHIITHGEEKD |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 67-166 of human MYL2 (P10916). |
Additional Info
| Tissue Specificity | Highly expressed in type I muscle fibers. |
| Post Translational Modifications | N-terminus is methylated by METTL11A/NTM1. Phosphorylated by MYLK3 and MYLK2.promotes cardiac muscle contraction and function. Dephosphorylated by PPP1CB complexed to PPP1R12B. The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation).regulates cardiac torsion and workload distribution. |
| Function | Contractile protein that plays a role in heart development and function. Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion.as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force. During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly. |
| Protein Name | Myosin Regulatory Light Chain 2 - Ventricular/Cardiac Muscle IsoformMlc-2Mlc-2vCardiac Myosin Light Chain 2Myosin Light Chain 2 - Slow Skeletal/Ventricular Muscle IsoformMlc-2s/VVentricular Myosin Light Chain 2 |
| Database Links | Reactome: R-HSA-390522 |
| Cellular Localisation | CytoplasmMyofibrilSarcomereA Band |
| Alternative Antibody Names | Anti-Myosin Regulatory Light Chain 2 - Ventricular/Cardiac Muscle Isoform antibodyAnti-Mlc-2 antibodyAnti-Mlc-2v antibodyAnti-Cardiac Myosin Light Chain 2 antibodyAnti-Myosin Light Chain 2 - Slow Skeletal/Ventricular Muscle Isoform antibodyAnti-Mlc-2s/V antibodyAnti-Ventricular Myosin Light Chain 2 antibodyAnti-MYL2 antibodyAnti-MLC2 antibody |
Information sourced from Uniprot.org