• Western blot analysis of extracts of mouse skeletal muscle, using MYH6/Alpha-MHC rabbit polyclonal antibody (STJ114830) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of mouse heart cells using MYH6/Alpha-MHC rabbit polyclonal antibody (STJ114830) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat heart cells using MYH6/Alpha-MHC rabbit polyclonal antibody (STJ114830) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse heart cells using MYH6/Alpha-MHC rabbit polyclonal antibody (STJ114830) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat heart cells using MYH6/Alpha-MHC rabbit polyclonal antibody (STJ114830) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MYH6 antibody (1-250) (STJ114830)

SKU:
STJ114830

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Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MYH6 (1-250) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYH6
Gene ID: 4624
Uniprot ID: MYH6_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human MYH6/Alpha-MHC (NP_002462.2).
Immunogen Sequence: MTDAQMADFGAAAQYLRKSE KERLEAQTRPFDIRTECFVP DDKEEFVKAKILSREGGKVI AETENGKTVTVKEDQVLQQN PPKFDKIEDMAMLTFLHEPA VLFNLKERYAAWMIYTYSGL FCVTVNPYKWLPVYNAEVVA AYRGKKRSEAPPHIFSISDN AYQYMLTDRENQSILITGES GAGKTVNTKRVIQYFASIAA IGDRGKKDNANANKGTLEDQ IIQANPALEAFGNAKTVRN
Function Muscle contraction.
Protein Name Myosin-6
Myosin Heavy Chain 6
Myosin Heavy Chain - Cardiac Muscle Alpha Isoform
Myhc-Alpha
Database Links Reactome: R-HSA-390522
Cellular Localisation Cytoplasm
Myofibril
Thick Filaments Of The Myofibrils
Alternative Antibody Names Anti-Myosin-6 antibody
Anti-Myosin Heavy Chain 6 antibody
Anti-Myosin Heavy Chain - Cardiac Muscle Alpha Isoform antibody
Anti-Myhc-Alpha antibody
Anti-MYH6 antibody
Anti-MYHCA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance