Western blot analysis of various lysates using MYL1 Rabbit pAb (STJ110736) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-MYL1 antibody (1-150) (STJ110736)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ110736
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General Information

Short DescriptionRabbit polyclonal MYL1 (1-150) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMYL1
Gene ID4632
Uniprot IDMYL1_HUMAN
Immunogen Region1-150
Immunogen SequenceMSFSADQIAEFKEAFLLFDR TGDSKITLSQVGDVLRALGT NPTNAEVRKVLGNPSNEELN AKKIEFEQFLPMMQAISNNK DQATYEDFVEGLRVFDKEGN GTVMGAELRHVLATLGEKMK EEEVEALMAGQEDSNGCINY EAFVKHIMSI
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human MYL1 (NP_524146.1).

Additional Info

Post Translational Modifications Isoform MLC3: Acetylated at position 2.
Function Non-regulatory myosin light chain required for proper formation and/or maintenance of myofibers, and thus appropriate muscle function.
Protein Name Myosin Light Chain 1/3 - Skeletal Muscle Isoform
Mlc1/Mlc3
Mlc1f/Mlc3f
Myosin Light Chain Alkali 1/2
Myosin Light Chain A1/A2
Database Links Reactome: R-HSA-390522
Cellular Localisation
Alternative Antibody Names Anti-Myosin Light Chain 1/3 - Skeletal Muscle Isoform antibody
Anti-Mlc1/Mlc3 antibody
Anti-Mlc1f/Mlc3f antibody
Anti-Myosin Light Chain Alkali 1/2 antibody
Anti-Myosin Light Chain A1/A2 antibody
Anti-MYL1 antibody

Information sourced from Uniprot.org

Citations

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