Metabolism Of RNA | Research Area | St John's Laboratory

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PP2A Catalytic Alpha-Y307 Rabbit mAb (STJ11102616) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-PPP2CA-Y307 antibody [S6MR] (STJ11102616)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€142.20 - €625.80

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Western blot analysis of lysates from HeLa cells, using Phospho-PKC alpha-S319 Rabbit pAb (STJ117877) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight or treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PRKCA-S319 antibody (STJ117877)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of various lysates using Phospho-PKC alpha-S657/Y658 Rabbit pAb (STJ11101090) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-PRKCA-S657/Y658 antibody (STJ11101090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€151.80 - €742.20

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Anti-Phospho-PRKCA-S657/Y658 antibody (STJA0005533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Human/Rabbit/Rat

€441.00

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Western blot analysis of 293T cells, using Phospho-PKC alpha-T638 antibody (STJ11105853) at 1:7000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-PRKCA-T638 antibody [S5853RM] (STJ11105853)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€142.20 - €625.80

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Western blot analysis of lysates from C2C12 cells, using Phospho-PKC alpha-T638 Rabbit mAb (STJ11102617) at 1:1000 dilution. C2C12 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PRKCA-T638 antibody [S7MR] (STJ11102617)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€142.20 - €625.80

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Western blot analysis of 3T3 cells using Phospho-PKC Alpha (T638) Polyclonal Antibody

Anti-Phospho-PRKCA-Thr638 antibody (606-655 aa) (STJ90863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PRKCA-Thr638/641 antibody (STJ196382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of 293 cells using Phospho-PKC Alpha (Y657) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PRKCA-Tyr657 antibody (623-672 aa) (STJ90499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PRKCD-Ser359 antibody (STJ196383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-PKC Delta (S645) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PRKCD-Ser645 antibody (612-661 aa) (STJ91011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

€58.80 - €313.20

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Western blot analysis of lysates from U-937 cells, using Phospho-PKC delta-T505 Rabbit pAb (STJ116396) at 1:2000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-PRKCD-T505 antibody (STJ116396)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€151.80 - €742.20

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Western blot analysis of 3T3 cells using Phospho-PKC Delta (T507) Polyclonal Antibody

Anti-Phospho-PRKCD-Thr507 antibody (471-520 aa) (STJ90388)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from HepG2 cells, COLO205 cells and HUVEC cells, using PKC delta (Phospho-Tyr313) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCD-Tyr313 antibody (279-328 aa) (STJ90864)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PKC delta (Phospho-Tyr52) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCD-Tyr52 antibody (18-67 aa) (STJ91068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using PKCD (Phospho-Tyr64) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCD-Tyr64 antibody (30-79 aa) (STJ90593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from U937 and Raw264. 7 cells, using Phospho-PKC delta-Y311 Rabbit pAb (STJ117870) at 1:1000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. RAW 264. 7 cells were treated by PMA (200 nM) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-PRKCD-Y311 antibody (STJ117870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€151.80 - €742.20

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Western blot analysis of 3T3 cells using Phospho-20S Proteasome Alpha 3 (S250) Polyclonal Antibody

Anti-Phospho-PSMA3-Ser250 antibody (206-255 aa) (STJ90556)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-RNA Polymerase II Subunit B1-Ser2 antibody [R06-5Y1] (STJA0036402)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Anti-Phospho-RNA Polymerase II Subunit B1-Ser5 antibody [R08-4O2] (STJA0036403)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Anti-Phospho-Rpb1 CTD-S2/S5-S2/S5 antibody [SRM15121] (STJ11109851)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ChIP/CUT&Tag/ELISA

Reactivity :

Human

€0.00

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Anti-Phospho-Rpb1-Ser2 antibody [PT1003R] (STJA0039928)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€342.00 - €648.00

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Anti-Phospho-Rpb1-Ser5 antibody [PT0923R] (STJA0039821)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Western blot analysis of lysates from HeLa cells using Phospho-S6 Ribosomal Protein (RPS6) -S235 Rabbit pAb (STJ11105772) at 1:800 dilution. HeLa cells were treated by 20% FBS at 37 °C for 90 minutes after serum starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-RPS6-S235 antibody (STJ11105772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Anti-Phospho-RPS6-S235-S235 antibody [SRM15136] (STJ11109866)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6) -S235/236 Rabbit pAb (STJ113489) at 1:1000 dilution. HeLa cells NIH/3T3 cells and PC-12 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-RPS6-S235/236 antibody (STJ113489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6) -S240/244 Rabbit pAb (STJ113488) at 1:1000 dilution or RPS6 antibody (STJ27852). HeLa and NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-RPS6-S240/244 antibody (STJ113488)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of lysates from 293 cells treated with serum 10% 15', using S6 Ribosomal Protein (Phospho-Ser235) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RPS6-Ser235 antibody (200-249 aa) (STJ90403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody. The lane on the right is blocked with the S6 Ribosomal Protein (Phospho-Ser235+Ser236) peptide.

Anti-Phospho-RPS6-Ser235/S236 antibody (200-249 aa) (STJ90992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunofluorescence analysis of HeLa cells, using S6 Ribosomal Protein (Phospho-Ser240) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RPS6-Ser240 antibody (200-249 aa) (STJ91053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-RPS6-Ser240/244 antibody [PT0447R] (STJA0039213)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Anti-Phospho-S6 Ribosomal Protein (RPS6)-S235/236-S235/236 antibody [SRM15117] (STJ11109847)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Anti-Phospho-S6 Ribosomal Protein (RPS6)-S235/236-S235/236 antibody [SRM15118] (STJ11109848)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Anti-Phospho-S6 Ribosomal Protein (RPS6)-S240/244-S240/244 antibody [SRM15119] (STJ11109849)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6) -S240/244 Rabbit mAb (STJ11107738) at 1:54000 dilution incubated overnight at 4C. HeLa, NIH/3T3 and PC-12 cells were treated by 10% FBS at 37C for 30 minutes after serum-starvation overnight. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 30s.

Anti-Phospho-S6 Ribosomal Protein-S240/244 antibody [SRM5547] (STJ11107738)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€172.20 - €742.20

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Western blot analysis of SH-SY5Y 293T using p-Splicing factor 1 (S82) antibody. Antibody was diluted at 1:500

Anti-Phospho-SF1-Ser82 antibody (48-97 aa) (STJ91054)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

€58.80 - €313.20

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Western blot analysis of various lysates using Phospho-SF3B1-T313 Rabbit pAb (STJ11100985) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-SF3B1-T313 antibody (STJ11100985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SF3B1-Thr313 antibody (STJ196415)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 15', using YB1 (Phospho-Ser102) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-YBX1-Ser102 antibody (68-117 aa) (STJ90649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from HeLa cells, using Phospho-YWHAZ-S58 Rabbit pAb (STJ22447). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-YWHAZ-S58 antibody (STJ22447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of various cells using Phospho-14-3-3 Zeta (S58) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-YWHAZ-Ser58 antibody (24-73 aa) (STJ91013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from Jurkat cells treated with UV 15', using 14-3-3 zeta/delta (Phospho-Thr232) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-YWHAZ-Thr232 antibody (196-245 aa) (STJ90869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from HepG2 cells, using TISB (Phospho-Ser92) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ZFP36L1-Ser92 antibody (58-107 aa) (STJ90549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-PKC Alpha antibody (Central Region) [M237] (STJA0041317)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IHC/IP

Reactivity :

Human/Mouse/Rat

€695.40

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Anti-PKC alpha antibody [SRM5132] (STJ11106955)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €665.40

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Anti-PKC Delta antibody (N-Term) [M242] (STJA0041318)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

€695.40

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Anti-PLRG1 antibody (STJA0007817)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various lysates using PNN Rabbit polyclonal antibody (STJ11102814) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-PNN antibody (1-100) (STJ11102814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€132.60 - €606.60

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