• Western blot analysis of lysates from 293 cells treated with serum 10% 15', using S6 Ribosomal Protein (Phospho-Ser235) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RPS6-Ser235 antibody (200-249 aa) (STJ90403)

SKU:
STJ90403

Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Small ribosomal subunit protein eS6-Ser235 (200-249 aa) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPS6
Gene ID: 6194
Uniprot ID: RS6_HUMAN
Immunogen Region: 200-249 aa
Specificity: Phospho-Ribosomal Protein S6 (S235) Polyclonal Antibody detects endogenous levels of Ribosomal Protein S6 protein only when phosphorylated at S235.
Immunogen: The antiserum was produced against synthesized peptide derived from the human S6 Ribosomal Protein around the phosphorylation site of Ser235 at the amino acid range 200-249
Function Component of the 40S small ribosomal subunit. Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Protein Name Small Ribosomal Subunit Protein Es6
40s Ribosomal Protein S6
Phosphoprotein Np33
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-166208
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6790901
Reactome: R-HSA-6791226
Reactome: R-HSA-72649
Reactome: R-HSA-72689
Reactome: R-HSA-72695
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-9725371
Reactome: R-HSA-9735869
Reactome: R-HSA-9754678
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Alternative Antibody Names Anti-Small Ribosomal Subunit Protein Es6 antibody
Anti-40s Ribosomal Protein S6 antibody
Anti-Phosphoprotein Np33 antibody
Anti-RPS6 antibody
Anti-OK antibody
Anti-SW-cl.2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance