• Western blot analysis of extracts of various cell lines, using Phospho-SF3B1-T313 antibody (STJ11100985) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using Phospho-SF3B1-T313 Rabbit polyclonal antibody (STJ11100985) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-SF3B1-T313 antibody (STJ11100985)

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STJ11100985

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-SF3B1-T313 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF3B1
Gene ID: 23451
Uniprot ID: SF3B1_HUMAN
Immunogen: A synthetic phosphorylated peptide around T313 of human SF3B1 (NP_036565.2).
Immunogen Sequence: AETPR
Post Translational Modifications Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis. Citrullinated by PADI4.
Function Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92. May also be involved in the assembly of the 'E' complex. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).
Protein Name Splicing Factor 3b Subunit 1
Pre-Mrna-Splicing Factor Sf3b 155 Kda Subunit
Sf3b155
Spliceosome-Associated Protein 155
Sap 155
Database Links Reactome: R-HSA-5250924
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Cellular Localisation Nucleus
Nucleus Speckle
During Mitosis
Transiently Dispersed From The Nuclear Speckles To The Cytoplasm
Alternative Antibody Names Anti-Splicing Factor 3b Subunit 1 antibody
Anti-Pre-Mrna-Splicing Factor Sf3b 155 Kda Subunit antibody
Anti-Sf3b155 antibody
Anti-Spliceosome-Associated Protein 155 antibody
Anti-Sap 155 antibody
Anti-SF3B1 antibody
Anti-SAP155 antibody

Information sourced from Uniprot.org

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