• Western blot analysis of lysates from PC-12 cells using Phospho-S6 Ribosomal Protein (RPS6) -S235 Rabbit polyclonal antibody (STJ11105772) at 1:800 dilution. PC-12 cells were treated by Anisomycin (25 ug/ml) at 37 °C for 30 minutes. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.
  • Western blot analysis of lysates from NIH/3T3 cells using Phospho-S6 Ribosomal Protein (RPS6) -S235 Rabbit polyclonal antibody (STJ11105772) at 1:800 dilution. NIH/3T3 cells were treated by 20% FBS at 37 °C for 90 minutes after serum starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.
  • Western blot analysis of lysates from HeLa cells using Phospho-S6 Ribosomal Protein (RPS6) -S235 Rabbit polyclonal antibody (STJ11105772) at 1:800 dilution. HeLa cells were treated by 20% FBS at 37 °C for 90 minutes after serum starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.

Anti-Phospho-RPS6-S235 antibody (STJ11105772)

SKU:
STJ11105772

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Host: Rabbit
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Small Ribosomal Subunit Protein Es6-S235 is suitable for use in research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Purification: Affinity purification
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPS6
Gene ID: 6194
Uniprot ID: RS6_HUMAN
Immunogen: A phospho specific peptide corresponding to residues surrounding S235 of human RPS6
Function Component of the 40S small ribosomal subunit. Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Protein Name Small Ribosomal Subunit Protein Es6
40s Ribosomal Protein S6
Phosphoprotein Np33
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-166208
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6790901
Reactome: R-HSA-6791226
Reactome: R-HSA-72649
Reactome: R-HSA-72689
Reactome: R-HSA-72695
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-9725371
Reactome: R-HSA-9735869
Reactome: R-HSA-9754678
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Alternative Antibody Names Anti-Small Ribosomal Subunit Protein Es6 antibody
Anti-40s Ribosomal Protein S6 antibody
Anti-Phosphoprotein Np33 antibody
Anti-RPS6 antibody
Anti-OK antibody
Anti-SW-cl.2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance