Gene Expression And Transcription | Research Area | St John's Laboratory

Immunofluorescence analysis of U-2 OS cells using Thioredoxin reductase 1 (TXNRD1 ) Rabbit polyclonal antibody (STJ119056) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TXNRD1 antibody (410-490) (STJ119056)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-TXNRD1 antibody [PT1432R] (STJA0040340)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g U2AF1 antibody (STJ115132). Western blot was performed from the immunoprecipitate using U2AF1 antibody (STJ115132) at a dilution of 1:1000.

Anti-U2AF1 antibody (1-100) (STJ115132)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-U2AF1 antibody (60-140 aa) (STJ190205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunoprecipitation analysis of 200 Mu g extracts of SW620 cells using 1 Mu g U2AF2/U2AF65 antibody (STJ26004). Western blot was performed from the immunoprecipitate using U2AF2/U2AF65 antibody (STJ26004) at a dilution of 1:500.

Anti-U2AF2 antibody (192-471) (STJ26004)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g U2AF2/U2AF65 antibody (STJ11102100). Western blot was performed from the immunoprecipitate using U2AF2/U2AF65 antibody (STJ11102100) at a dilution of 1:1000.

Anti-U2AF2 antibody (376-475) [S0MR] (STJ11102100)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Anti-U2AF2 antibody (STJA0007037)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-U2AF2 antibody [R09-1V1] (STJA0036879)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Immunocytochemistry analysis of Ubiquitin 60S Ribosomal Protein L40 (green) in Hela using Ubiquitin 60S Ribosomal Protein L40 antibody, and DAPI (blue).

Anti-UBA52 antibody (39-52 aa) [R05-5D6] (STJA0012632)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

$184.23 - $388.47

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-UBA52 antibody (71-120 aa) (STJ95481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Ub antibody at 2500 dilution; cells stimulated with MG132; lysate at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-UBA52 antibody (STJ140085)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

$130.41 - $607.89

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Anti-UBA52 antibody [PT0081R] (STJA0039231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Anti-UBA52 antibody [SRM13090] (STJ11107820)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Confocal imaging of MCF7 cells using K48-linkage Specific Ubiquitin Rabbit monoclonal antibody (STJ11101909, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-UBB antibody (1-100) [S9MR] (STJ11101909)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of extracts of various cell lines, using Ubiquitin antibody (STJ11101766) at1:1000 dilution. HeLa, NIH/3T3 and C6 cell were treated by MG132 (50 Mu M) at 37 °C for 90 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-UBB antibody (1-228) [S6MR] (STJ11101766)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

$198.03 - $853.53

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Immunofluorescence analysis of HeLa cells using Ubiquitin Rabbit polyclonal antibody (STJ11100145) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-UBB antibody (1-229) (STJ11100145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$152.49 - $697.59

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Western blot analysis of lysates from C6 cells using Ubiquitin Rabbit polyclonal antibody (STJ26009) at 1:900 dilution. C6 cells were treated by MG132 (50 Mu M) at 37 °C for 90 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-UBB antibody (1-229) (STJ26009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of HepG2 cells using UBC Rabbit polyclonal antibody (STJ29897) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-UBC antibody (1-100) (STJ29897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-UBC antibody (160-210 aa) (STJ194141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-UBC/polyUb/Polyubiquitin-C antibody [SAA0328] (STJA0019255)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/IP/WB

Reactivity :

Human

$269.79 - $1,820.91

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Western blot analysis of lysates from wild type (WT) and UBE2C knockout (KO) HeLa cells, using [KO Validated] UBE2C Rabbit polyclonal antibody (STJ11100048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBE2C antibody (1-179) (STJ11100048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$174.57 - $853.53

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Western blot analysis of various lysates using UBE2C Rabbit polyclonal antibody (STJ11104295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBE2C antibody (1-179) (STJ11104295)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of various lysates, using UBE2C antibody (STJ27450) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-UBE2C antibody (1-179) (STJ27450)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of U-2 OS cells using UBE2C Rabbit monoclonal antibody (STJ11102292) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-UBE2C antibody (70-150) [S2MR] (STJ11102292)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$174.57 - $765.21

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Anti-UBE2C antibody (STJA0007012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-UBE2C antibody [R06-4V-3] (STJA0033078)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

$296.01 - $425.73

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Anti-UBE2C antibody [SRM14772] (STJ11109502)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using UBE2D1 antibody (STJ26014) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-UBE2D1 antibody (1-147) (STJ26014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using SFT antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-UBE2D1 antibody (119-132 aa) [R07-4E8] (STJA0012589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

$184.23 - $388.47

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Immunohistochemistry analysis of SFT in paraffin-embedded rat ovary using SFT Rabbit monoclonal antibody (STJ11104166) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-UBE2D1 antibody (50-147) [S4166RM] (STJ11104166)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-UBE2D1 antibody (STJ191590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of various lysates using UBE2D3 Rabbit polyclonal antibody (STJ116847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-UBE2D3 antibody (1-100) (STJ116847)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-UBE2D3 antibody (STJ191591)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysates using UBE2E1 Rabbit polyclonal antibody (STJ11101172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBE2E1 antibody (1-50) (STJ11101172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-UBE2E1 antibody (STJA0009631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using UBE2I rabbit monoclonal antibody (STJ11102069) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-UBE2I antibody (1-100) [S9MR] (STJ11102069)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunohistochemistry analysis of paraffin-embedded rat lung using UBE2I Rabbit polyclonal antibody (STJ26017) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-UBE2I antibody (1-158) (STJ26017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-UBE2I antibody (STJA0010235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis using UBC9 monoclonal antibody against HeLa (1) , HepG2 (2) , and Cos7 (3) cell lysate.

Anti-UBE2I antibody [1B10] (STJ98444)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

$205.62 - $496.80

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Western blot analysis of various lysates using UBE2S Rabbit pAb (STJ26022) at 1:1000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 15s.

Anti-UBE2S antibody (1-222) (STJ26022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from wild type (WT) and UBE2S knockout (KO) 293T cells, using [KO Validated] UBE2S Rabbit polyclonal antibody (STJ11100037) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-UBE2S antibody (122-221) (STJ11100037)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-UBE2S antibody (STJ191602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-Ubiquitin antibody (STJA0040904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ICC/WB

Reactivity :

Human/Mouse/Rat

$729.33

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Anti-Ubiquitin antibody [1B8-8E7-1H7] (STJA0032907)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Transfected

$244.95 - $360.87

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Anti-Ubiquitin antibody [7F2-4B1-10D3] (STJA0032914)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Transfected

$184.23 - $388.47

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Anti-Ubiquitin antibody [R01-7F1] (STJA0034973)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Rat

$184.23 - $388.47

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Anti-Ubiquitin antibody [R02-4I6] (STJA0034974)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

$184.23 - $388.47

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Anti-Ubiquitin antibody [SRM13312] (STJ11108042)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$0.00

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