Anti-U2AF2 antibody [R09-1V1] (STJA0036879)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human U2AF65
General Information
| Short Description | Rabbit monoclonal anti-U2AF2 for use in WB, IHC-P, ICC and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R09-1V1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | U2AF2 |
| Gene ID | 11338 |
| Uniprot ID | U2AF2_HUMAN |
| Immunogen | A synthesized peptide derived from human U2AF65 |
Additional Info
| Post Translational Modifications | Lysyl-hydroxylation at Lys-15 and Lys-276 affects the mRNA splicing activity of the protein, leading to regulate some, but not all, alternative splicing events. |
| Function | Plays a role in pre-mRNA splicing and 3'-end processing. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals. |
| Protein Name | Splicing Factor U2af 65 Kda SubunitU2 Auxiliary Factor 65 Kda SubunitHu2af(65Hu2af65U2 Snrnp Auxiliary Factor Large Subunit |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72163Reactome: R-HSA-72187Reactome: R-HSA-73856Reactome: R-HSA-9629569 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Splicing Factor U2af 65 Kda Subunit antibodyAnti-U2 Auxiliary Factor 65 Kda Subunit antibodyAnti-Hu2af(65 antibodyAnti-Hu2af65 antibodyAnti-U2 Snrnp Auxiliary Factor Large Subunit antibodyAnti-U2AF2 antibodyAnti-U2AF65 antibody |
Information sourced from Uniprot.org