Anti-U2AF2 antibody (STJA0007037)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human U2AF65
General Information
| Short Description | Rabbit polyclonal anti-Splicing factor U2AF 65 kDa subunit for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | U2AF2 |
| Gene ID | 11338 |
| Uniprot ID | U2AF2_HUMAN |
| Immunogen | Synthesized peptide derived from human U2AF65 |
| Specificity | This antibody detects endogenous levels of U2AF65 at Human, Mouse |
Additional Info
| Function | Plays a role in pre-mRNA splicing and 3'-end processing. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals. |
| Protein Name | Splicing Factor U2af 65 Kda SubunitU2 Auxiliary Factor 65 Kda SubunitHu2af(65Hu2af65U2 Snrnp Auxiliary Factor Large Subunit |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72163Reactome: R-HSA-72187Reactome: R-HSA-73856Reactome: R-HSA-9629569 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Splicing Factor U2af 65 Kda Subunit antibodyAnti-U2 Auxiliary Factor 65 Kda Subunit antibodyAnti-Hu2af(65 antibodyAnti-Hu2af65 antibodyAnti-U2 Snrnp Auxiliary Factor Large Subunit antibodyAnti-U2AF2 antibodyAnti-U2AF65 antibody |
Information sourced from Uniprot.org