| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Ubiquitin-conjugating enzyme E2 C E2 ubiquitin-conjugating enzyme C is suitable for use in Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | UBE2C |
| Gene ID: | 11065 |
| Uniprot ID: | UBE2C_HUMAN |
| Specificity: | This antibody detects endogenous levels of UBE2C at Human, Mouse |
| Immunogen: | Synthesized peptide derived from human UBE2C |
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 C(E3-Independent E2 Ubiquitin-Conjugating Enzyme CE2 Ubiquitin-Conjugating Enzyme CUbch10Ubiquitin Carrier Protein CUbiquitin-Protein Ligase C |
| Database Links | Reactome: R-HSA-141430Reactome: R-HSA-174048Reactome: R-HSA-174084Reactome: R-HSA-174154Reactome: R-HSA-174178Reactome: R-HSA-174184Reactome: R-HSA-176407Reactome: R-HSA-176408Reactome: R-HSA-176409Reactome: R-HSA-176412Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2559582Reactome: R-HSA-68867Reactome: R-HSA-69017Reactome: R-HSA-8853884Reactome: R-HSA-8866652Reactome: R-HSA-9687136Reactome: R-HSA-983168 |
| Alternative Antibody Names | Anti-Ubiquitin-Conjugating Enzyme E2 C antibodyAnti-(E3-Independent E2 Ubiquitin-Conjugating Enzyme C antibodyAnti-E2 Ubiquitin-Conjugating Enzyme C antibodyAnti-Ubch10 antibodyAnti-Ubiquitin Carrier Protein C antibodyAnti-Ubiquitin-Protein Ligase C antibodyAnti-UBE2C antibodyAnti-UBCH10 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and UBE2C knockout (KO) HeLa cells, using [KO Validated] UBE2C Rabbit polyclonal antibody (STJ11100048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89120/357572/STJ11100048_1__15924.1713121701.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and UBE2C knockout (KO) HeLa cells, using [KO Validated] UBE2C Rabbit polyclonal antibody (STJ11100048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")