| Host: |
Rabbit |
| Applications: |
WB/IF/IP |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-U2AF2 (192-471) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:100IP 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
U2AF2 |
| Gene ID: |
11338 |
| Uniprot ID: |
U2AF2_HUMAN |
| Immunogen Region: |
192-471 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 192-471 of human U2AF2/U2AF65 (NP_001012496.1). |
| Immunogen Sequence: |
NQDKNFAFLEFRSVDETTQA MAFDGIIFQGQSLKIRRPHD YQPLPGMSENPSVYVPGVVS TVVPDSAHKLFIGGLPNYLN DDQVKELLTSFGPLKAFNLV KDSATGLSKGYAFCEYVDIN VTDQAIAGLNGMQLGDKKLL VQRASVGAKNATLSTINQTP VTLQVPGLMSSQVQMGGHPT EVLCLMNMVLPEELLDDEEY EEIVEDVRDECSKYGLVKSI EIPRPVDGVEVPGCGKIFV |
| Post Translational Modifications | Lysyl-hydroxylation at Lys-15 and Lys-276 affects the mRNA splicing activity of the protein, leading to regulate some, but not all, alternative splicing events. |
| Function | Plays a role in pre-mRNA splicing and 3'-end processing. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals. |
| Protein Name | Splicing Factor U2af 65 Kda SubunitU2 Auxiliary Factor 65 Kda SubunitHu2af(65Hu2af65U2 Snrnp Auxiliary Factor Large Subunit |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72163Reactome: R-HSA-72187Reactome: R-HSA-73856 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Splicing Factor U2af 65 Kda Subunit antibodyAnti-U2 Auxiliary Factor 65 Kda Subunit antibodyAnti-Hu2af(65 antibodyAnti-Hu2af65 antibodyAnti-U2 Snrnp Auxiliary Factor Large Subunit antibodyAnti-U2AF2 antibodyAnti-U2AF65 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunofluorescence analysis of MCF7 cells using [KO Validated] MMP13 Rabbit polyclonal antibody (STJ119232) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98104/381734/STJ119232_1__37752.1713149924.jpg?c=1 "Immunofluorescence analysis of MCF7 cells using [KO Validated] MMP13 Rabbit polyclonal antibody (STJ119232) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")