Anti-U2AF2 antibody (376-475) [S0MR] (STJ11102100)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal U2AF65 (376-475) antibody for use in WB, IHC-P, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S0MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000IHC-P:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requiremen |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | U2AF2 |
| Gene ID | 11338 |
| Uniprot ID | U2AF2_HUMAN |
| Immunogen Region | 376-475 |
| Immunogen Sequence | EVLCLMNMVLPEELLDDEEY EEIVEDVRDECSKYGLVKSI EIPRPVDGVEVPGCGKIFVE FTSVFDCQKAMQGLTGRKFA NRVVVTKYCDPDSYHRRDFW |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 376-475 of human U2AF2/U2AF2/U2AF65 (P26368). |
Additional Info
| Post Translational Modifications | Lysyl-hydroxylation at Lys-15 and Lys-276 affects the mRNA splicing activity of the protein, leading to regulate some, but not all, alternative splicing events. |
| Function | Plays a role in pre-mRNA splicing and 3'-end processing. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals. |
| Protein Name | Splicing Factor U2af 65 Kda SubunitU2 Auxiliary Factor 65 Kda SubunitHu2af(65Hu2af65U2 Snrnp Auxiliary Factor Large Subunit |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72163Reactome: R-HSA-72187Reactome: R-HSA-73856Reactome: R-HSA-9629569 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Splicing Factor U2af 65 Kda Subunit antibodyAnti-U2 Auxiliary Factor 65 Kda Subunit antibodyAnti-Hu2af(65 antibodyAnti-Hu2af65 antibodyAnti-U2 Snrnp Auxiliary Factor Large Subunit antibodyAnti-U2AF2 antibodyAnti-U2AF65 antibody |
Information sourced from Uniprot.org