• Western blot analysis of various lysates using UBE2C Rabbit polyclonal antibody (STJ11104295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBE2C antibody (1-179) (STJ11104295)

SKU:
STJ11104295

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ubiquitin-Conjugating Enzyme E2 C (1-179) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE2C
Gene ID: 11065
Uniprot ID: UBE2C_HUMAN
Immunogen Region: 1-179
Immunogen: Recombinant Protein corresponding to a sequence within amino acids 1-179 of human UBE2C (NP_008950.1).
Immunogen Sequence: MASQNRDPAATSVAAARKGA EPSGGAARGPVGKRLQQELM TLMMSGDKGISAFPESDNLF KWVGTIHGAAGTVYEDLRYK LSLEFPSGYPYNAPTVKFLT PCYHPNVDTQGNICLDILKE KWSALYDVRTILLSIQSLLG EPNIDSPLNTHAAELWKNPT AFKKYLQETYSKQVTSQEP
Post Translational Modifications Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
Protein Name Ubiquitin-Conjugating Enzyme E2 C
(E3-Independent E2 Ubiquitin-Conjugating Enzyme C
E2 Ubiquitin-Conjugating Enzyme C
Ubch10
Ubiquitin Carrier Protein C
Ubiquitin-Protein Ligase C
Database Links Reactome: R-HSA-141430
Reactome: R-HSA-174048
Reactome: R-HSA-174084
Reactome: R-HSA-174154
Reactome: R-HSA-174178
Reactome: R-HSA-174184
Reactome: R-HSA-176407
Reactome: R-HSA-176408
Reactome: R-HSA-176409
Reactome: R-HSA-176412
Reactome: R-HSA-179409
Reactome: R-HSA-2467813
Reactome: R-HSA-2559582
Reactome: R-HSA-68867
Reactome: R-HSA-69017
Reactome: R-HSA-8853884
Reactome: R-HSA-8866652
Reactome: R-HSA-9687136
Reactome: R-HSA-983168
Alternative Antibody Names Anti-Ubiquitin-Conjugating Enzyme E2 C antibody
Anti-(E3-Independent E2 Ubiquitin-Conjugating Enzyme C antibody
Anti-E2 Ubiquitin-Conjugating Enzyme C antibody
Anti-Ubch10 antibody
Anti-Ubiquitin Carrier Protein C antibody
Anti-Ubiquitin-Protein Ligase C antibody
Anti-UBE2C antibody
Anti-UBCH10 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance